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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 44-1028G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Phospho-Paxillin (Ser273) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Lot Dependent
- Storage
- -20°C
Submitted references Paxillin phosphorylation counteracts proteoglycan-mediated inhibition of axon regeneration.
Paxillin phosphorylation at Ser273 localizes a GIT1-PIX-PAK complex and regulates adhesion and protrusion dynamics.
Kuboyama T, Luo X, Park K, Blackmore MG, Tojima T, Tohda C, Bixby JL, Lemmon VP, Kamiguchi H
Experimental neurology 2013 Oct;248:157-69
Experimental neurology 2013 Oct;248:157-69
Paxillin phosphorylation at Ser273 localizes a GIT1-PIX-PAK complex and regulates adhesion and protrusion dynamics.
Nayal A, Webb DJ, Brown CM, Schaefer EM, Vicente-Manzanares M, Horwitz AR
The Journal of cell biology 2006 May 22;173(4):587-9
The Journal of cell biology 2006 May 22;173(4):587-9
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Antibody Specificity. Lysates prepared from Calyculin-treated CHO-K1 transfected with vector alone (1), wild type Paxillin (2, 5-8), mutant Paxillin S273A (3), or mutant Paxillin S273D (4) were resolved on a 10% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 3% BSA-TBST buffer for one hour at room temperature, and then incubated with the Paxillin (pS273) antibody for two hours at room temperature in 3% BSA-TBST buffer, following prior incubation with: no peptide (5), the non-phosphopeptide corresponding to the phosphopeptide immunogen (6), a generic phosphoserine-containing peptide (7), or the phosphopeptide immunogen (8). After washing, the membrane was incubated with goat F (ab’)2 anti-rabbit IgG HRP conjugate (Product # ALI4404) and signals were detected using the Pierce SuperSignal™ method. The data show that only the phosphopeptide corresponding to Paxillin (pS273) blocks this signal and that the signal is abolished in mutant S273A and S273D, indicating that the signal is phosphorylation site-specific.
