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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX633678 - Provider product page
- Provider
- GeneTex
- Product name
- Paxillin (phospho Tyr118) antibody [GT162]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) A431 whole cell extracts (5 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT162] (GTX633678) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT162] (GTX633678) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) A431 whole cell extracts (5 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT162] (GTX633678) diluted at 1:3000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT162] (GTX633678) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Paxillin (phospho Tyr118) antibody [GT162] detects Paxillin (phospho Tyr118) protein at focal adhesion sites by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Paxillin (phospho Tyr118) protein stained by Paxillin (phospho Tyr118) antibody [GT162] (GTX633678) diluted at 1:200.Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
