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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PAB7932 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7932, RRID:AB_1710820
- Product name
- Pxn (phospho S83) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of Pxn.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Paxillin serves as an ERK-regulated scaffold for coordinating FAK and Rac activation in epithelial morphogenesis.
Phosphorylation of paxillin by p38MAPK is involved in the neurite extension of PC-12 cells.
Ishibe S, Joly D, Liu ZX, Cantley LG
Molecular cell 2004 Oct 22;16(2):257-67
Molecular cell 2004 Oct 22;16(2):257-67
Phosphorylation of paxillin by p38MAPK is involved in the neurite extension of PC-12 cells.
Huang C, Borchers CH, Schaller MD, Jacobson K
The Journal of cell biology 2004 Feb 16;164(4):593-602
The Journal of cell biology 2004 Feb 16;164(4):593-602
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PC-12 cells untreated (lane 1) or treated with NGF (200 ng/ml) for 60 min (lane 2). The blots were probed with Pxn (phospho S83) polyclonal antibody (Cat # PAB7932).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunocytochemical labeling of Pxn (phospho S83) polyclonal antibody (Cat # PAB7932) in rabbit spleen fibroblasts. The cells were labeled with Pxn (phospho S83) polyclonal antibody (Cat # PAB7932) (right) , then detected using appropriate secondary antibodies conjugated to Cy3.
- Validation comment
- Immunofluorescence
