Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AP09394PU-N - Provider product page
- Provider
- OriGene
- Product name
- PKC beta 1 (PRKCB) rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- PKC beta 1 (PRKCB) rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PRKCB
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-PKC beta antibody shows detection of PKC beta in ~25 ug of U251 whole cell lysate (glioma derived). A 4-12% gel was used for separation. The arrowhead corresponds to 76 kDa PKC beta. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BSA in TTBS at 4¡ã C, overnight.
- Validation comment
- WB