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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB4532 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4532, RRID:AB_1579132
- Product name
- PRKCB polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PRKCB.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A chromatin immunoprecipitation screen reveals protein kinase Cbeta as a direct RUNX1 target gene.
Centrosomal anchoring of protein kinase C betaII by pericentrin controls microtubule organization, spindle function, and cytokinesis.
Protein kinase C-beta-mediated complex formation between tyrosinase and TRP-1.
Hug BA, Ahmed N, Robbins JA, Lazar MA
The Journal of biological chemistry 2004 Jan 9;279(2):825-30
The Journal of biological chemistry 2004 Jan 9;279(2):825-30
Centrosomal anchoring of protein kinase C betaII by pericentrin controls microtubule organization, spindle function, and cytokinesis.
Chen D, Purohit A, Halilovic E, Doxsey SJ, Newton AC
The Journal of biological chemistry 2004 Feb 6;279(6):4829-39
The Journal of biological chemistry 2004 Feb 6;279(6):4829-39
Protein kinase C-beta-mediated complex formation between tyrosinase and TRP-1.
Wu H, Park HY
Biochemical and biophysical research communications 2003 Nov 28;311(4):948-53
Biochemical and biophysical research communications 2003 Nov 28;311(4):948-53
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PRKCB polyclonal antibody (Cat # PAB4532) in placenta lysate . PRKCB (arrow) was detected using purified PRKCB polyclonal antibody (Cat # PAB4532) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKCB polyclonal antibody (Cat # PAB4532) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
