711723

antibody from Invitrogen Antibodies

Targeting: PRKCB PKCB, PKCβ, PRKCB1, PRKCB2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

711723

Provider

Invitrogen Antibodies

Product name

Phospho-PKC beta-1 (Thr642) Recombinant Polyclonal Antibody (23HCLC)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

23HCLC

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on Whole cell extracts (30 µg lysate) of K-562 (Lane 1) and K-562 treated with PMA (1 ng/mL for 10 mins) (Lane 2). The blots were probed with Anti-PKC BETA I (pT 642) Recombinant Rabbit Polyclonal Antibody (Product # 711723, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 77 kDa band corresponding to PKC BETA I (pT 642) was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).