Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [6]
- Flow cytometry [1]
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Validation data
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- Product number
- RQ4656 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PKC beta 1 Antibody / PRKCB1
- Antibody type
- Polyclonal
- Description
- This highly specific PKC beta 1 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the PKC beta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) K562, 2) THP-1, 3) HEK293, 4) Jurkat, 5) Raji, 6) HL-60 and 7) A549 lysate with PKC beta antibody at 0.5ug/ml. Predicted molecular weight ~76 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of rat 1) thymus, 2) spleen, 3) brain, 4) C6 cells, and mouse 5) thymus, 6) spleen, 7) brain, 8) Neuro-2a cells and 9) NIH 3T3 cell lysate with PKC beta antibody at 0.5ug/ml. Predicted molecular weight ~76 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human K562, 2) human HEL, 3) human Jurkat, 4) rat brain, 5) rat PC-12 and 6) mouse brain tissue lysate with PKC beta antibody. Predicted molecular weight ~76 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human intestinal cancer with PKC beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human prostate cancer with PKC beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human placenta with PKC beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse small intestine with PKC beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat lung with PKC beta antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse brain tissue with PKC beta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human Jurkat cells with PKC beta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PKC beta antibody.