Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- Ab122050 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant PKC beta 2 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human PKC beta 2 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on.Application:WB: 1/500-1/1000IHC: 1/50-1/100ICC/IF: 1/50-1/200Protein FunctionCalcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 170-350
- Epitope
- reacts with an epitope located in the region near the C terminus of PKC beta 2.
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,5 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant PKC beta 2 Antibody (Ab122050) - Western Blot All lanes: Recombinant PKC beta 2 Antibody (Ab122050) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution Predicted band size: 80 kDa Observed band size: 79 kDa Exposure time: 2.1 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant PKC beta 2 Antibody (Ab122050) - ICC/IF Immunocytochemistry analysis of beta Tubulin(green) in K562 cells using Recombinant PKC beta 2 Antibody (Ab122050) and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant PKC beta 2 Antibody (Ab122050) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant PKC beta 2 Antibody (Ab122050). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.