PA5-29680

antibody from Invitrogen Antibodies

Targeting: PRKCB PKCB, PKCβ, PRKCB1, PRKCB2

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Antibody data

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Validation data

Product number: PA5-29680 - Provider product page
Provider: Invitrogen Antibodies
Product name: PKC beta Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: SK-N-SH, IMR32, SK-N-AS. Predicted reactivity: Mouse (98%), Rat (98%), Rabbit (98%), Rhesus Monkey (100%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat, Xenopus
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.48 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

PRKCB protein structure - PA5-29680 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PKC beta using 30 µg of IMR32 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PKC beta polyclonal antibody (Product # PA5-29680) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of PKC-beta-1 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PKC beta-1 Polyclonal Antibody (Product # PA5-29680) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using PKC beta Polyclonal Antibody (Product # PA5-29680). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC beta Polyclonal Antibody (Product # PA5-29680) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1), U-87 MG (Lane 2), SK-N-AS (Lane 3), IMR-32 (Lane 4) and tissue extract of Mouse Brain (Lane 5). The blot was probed with Anti-PKC beta-1 Polyclonal Antibody (Product # PA5-29680, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 77 kDa band corresponding to PKC beta-1 was observed across the cell lines and tissue tested.