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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AP09394PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09394PU-S, RRID:AB_2035859
- Product name
- anti PRKCB
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to an internal region of human Protein kinase C beta
- Reactivity
- Human, Mouse, Rat, Bovine, Rabbit, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µl
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-PKC beta antibody shows detection of PKC beta in ~25 µg of U251 whole cell lysate (glioma derived). A 4-12% gel was used for separation. The arrowhead corresponds to 76 kDa PKC beta. The membrane was probed with the primary antibody at a 1:1,000 dilution in 5% BSA in TTBS at 4° C, overnight.
