GTX25782

antibody from GeneTex

Targeting: PRKCB PKCB, PKCβ, PRKCB1, PRKCB2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX25782

Provider

GeneTex

Proper citation

GeneTex Cat#GTX25782, RRID:AB_380683

Product name

PKC beta 1 (phospho Thr642) antibody

Antibody type

Polyclonal

Reactivity

Human, Rat

Host

Rabbit

References

Submitted references

Activation of PKC isoform beta(I) at the blood-brain barrier rapidly decreases P-glycoprotein activity and enhances drug delivery to the brain.

Rigor RR, Hawkins BT, Miller DS
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2010 Jul;30(7):1373-83

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Lysates prepared from K562 cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with PKCbetaI [pT642] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG HRP conjugate and bands were detected. The data show that only the peptide corresponding to PKCbetaI [pT642] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.