18244-1-AP

antibody from Proteintech Group

Targeting: EIF2AK2 EIF2AK1, PKR, PPP1R83, PRKR

Provider product page for 18244-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [62]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 18244-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#18244-1-AP, RRID:AB_2246451
Product name: EIF2AK2, PKR antibody
Antibody type: Polyclonal
Description: KD/KO validated EIF2AK2, PKR antibody (Cat. #18244-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse , rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

EIF2AK2 protein structure - 18244-1-AP shown in red.

Submitted references ATG16L1 Regulates Reparative Function of Peritoneal Macrophages During Acute Drug-induced Liver Injury.

Wang X, Zhan X, Gao Y, Wang H, Liu Z, Liu M, Lu L, Zhou H
Cellular and molecular gastroenterology and hepatology 2026;20(2):101674

Ageing promotes metastasis via activation of the integrated stress response.

Patel AAH, Dzanan JJ, Ali KX, Eklund EA, Alvarez SW, Raj D, Dankis M, Altinönder I, Schwarz M, Le Gal K, Bedel E, El Zowalaty AE, Jonasson E, Albatrok H, Gul N, Bossowski JP, Pillai R, Micke P, Botling J, Akyürek LM, Angeletti D, Sayin SI, Härtlova A, Papagiannakopoulos T, Olofsson Bagge R, Ståhlberg A, Hallqvist A, Wiel C, Sayin VI
Nature 2026 Apr;652(8112):1339-1348

CAV2 Modulates Cetuximab Sensitivity in HNSCC via Ubiquitin-Mediated Disruption of the PACT-PKR Axis.

Wang Y, Wang Y, Yuan D, Liu S, Chen P
Cancers 2026 Apr 2;18(7)

eIF3f promotes tumour malignancy by remodelling fatty acid biosynthesis in hepatocellular carcinoma.

Zhou S, Zhang L, You Y, Yu K, Tie X, Gao Y, Chen Y, Yao F, Zhang R, Hao X, Fang C, Li X, Li Q, Wang X
Journal of hepatology 2025 Sep;83(3):712-728

Identification of autophagy-related biomarkers in the circulatory system of ischemic stroke.

Xu Y, Duan J, Zhong D, Yuan M, Zhang G, Zhu Z, Xie Y
Neurological research 2025 Sep 2;:1-18

Effect of berberine on herpes simplex keratitis through the dimerization of eukaryotic translation initiation factor 2-alpha kinase 2.

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Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Oct;146:157112

Apolipoprotein B100 acts as a tumor suppressor in ovarian cancer via lipid/ER stress axis-induced blockade of autophagy.

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Acta pharmacologica Sinica 2025 May;46(5):1445-1461

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Communications medicine 2025 Jul 10;5(1):286

Induction of translation-suppressive G3BP1(+) stress granules and interferon-signaling cGAS condensates by transfected plasmid DNA.

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hLife 2025 Jan;3(1):21-37

Early Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Infection Induces Necroptosis in Immune Cells of Peripheral Lymphoid Organs.

Xu J, Huo C, Yang Y, Han J, Zhou L, Hu Y, Yang H
Viruses 2025 Feb 20;17(3)

Cardiovirus-Mediated PKR inhibition results from nucleocytoplasmic trafficking disruption.

Milcamps R, Lizcano-Perret B, Wavreil F, Lebrun M, Aloise C, Vertommen D, Herinckx G, van Kuppeveld FJM, Sadzot C, Michiels T
PLoS pathogens 2025 Dec;21(12):e1013420

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eLife 2025 Aug 29;14

Characterization of dsRNA binding proteins through solubility analysis identifies ZNF385A as a dsRNA homeostasis regulator.

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Nature communications 2025 Apr 11;16(1):3433

African swine fever virus RNA polymerase subunits C315R and H359L inhibition host translation by activating the PKR-eIF2a pathway and suppression inflammatory responses.

Yang S, Wang Y, Yang J, Tian Z, Wu M, Sun H, Zhang X, Zhao Y, Luo J, Guan G, Yin H, Hao R, Niu Q
Frontiers in microbiology 2024;15:1469166

Long non-coding RNA GRASLND links melanoma differentiation and interferon-gamma response.

Fischer KD, Tiwari S, Thier B, Qiu LC, Lin TC, Paschen A, Imig J
Frontiers in molecular biosciences 2024;11:1471100

Single-cell RNA-sequencing analysis reveals α-syn induced astrocyte-neuron crosstalk-mediated neurotoxicity.

Li K, Ling H, Huang W, Luo W, Gu C, Tao B, Xie Q, Qiu P
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Blockade of pan-viral propagation by inhibition of host cell PNPT1.

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International journal of antimicrobial agents 2024 May;63(5):107124

African swine fever virus maintains de novo global cellular protein synthesis and inhibits stress granules formation via dephosphorylating eIF2α.

Gao H, Gao X, Liu X, Luo Y, Zhong J, Liu J, Yan L, Wang H, Gong L, Zhang G, Zheng Z, Sun Y
Veterinary microbiology 2024 Mar;290:109988

Identification and Validation of PKR as a Direct Target for the Novel Sulfonamide-Substituted Tetrahydroquinoline Nonselective Inhibitor of the NLRP3 Inflammasome.

Yang F, Dai Z, Xue MY, Chen XY, Liu J, Wang L, Xu LL, Di B
Journal of medicinal chemistry 2024 Jun 27;67(12):10168-10189

Deubiquitylase OTUD3 regulates integrated stress response to suppress progression and sorafenib resistance of liver cancer.

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Cell reports 2024 Jul 23;43(7):114487

Zearalenone modulates the function of goat endometrial cells via the mitochondrial quality control system.

Zhang G, Zeng C, Sun X, Zhang Q, Wang Y, Xia R, Mai Q, Xue G, Huang H, Wang F
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Jul 15;38(13):e23701

Long non-coding RNA SUN2-AS1 acts as a negative regulator of ISGs transcription to promote flavivirus infection.

Yang C, Chen W, Huang Y
Virology 2024 Dec;600:110245

Knockdown of EIF2AK2-OAS1 axis reduces ATP production inducing AMPK phosphorylation to inhibit the malignant behavior of gastric cancer cells.

Lai Y, Wang X, Ma J, Du C, Wang Y, Wang Y, Yuan W, Zhao M
Journal of bioenergetics and biomembranes 2024 Aug;56(4):433-449

A testis-specific lncRNA functions as a post-transcriptional regulator of MDM2 and stimulates apoptosis of testicular germ cell tumor cells.

Ito S, Ueno A, Ueda T, Ogura R, Sako S, Gabata Y, Murashita J, Takahashi H, Ukimura O
Cell death discovery 2024 Aug 3;10(1):348

Calreticulin regulates the expression of MMP14 and ADAR1 through EIF2AK2 signaling to promote the proliferation and progression of malignant melanoma cells.

Liang L, Wang J, Guo T, Huang L, Wu Y, Xu R, Huang T, Ma B
Neoplasma 2024 Apr;71(2):180-192

Hyperoside inhibits EHV-8 infection via alleviating oxidative stress and IFN production through activating JNK/Keap1/Nrf2/HO-1 signaling pathways.

Wang T, Hu L, Li R, Ren H, Li S, Sun Q, Ding X, Li Y, Wang C, Li L
Journal of virology 2024 Apr 16;98(4):e0015924

Human herpesvirus 8 ORF57 protein is able to reduce TDP-43 pathology: network analysis identifies interacting pathways.

Webber CJ, Murphy CN, Rondón-Ortiz AN, van der Spek SJF, Kelly EX, Lampl NM, Chiesa G, Khalil AS, Emili A, Wolozin B
Human molecular genetics 2023 Oct 4;32(20):2966-2980

Virus-induced lncRNA-BTX allows viral replication by regulating intracellular translocation of DHX9 and ILF3 to induce innate escape.

Cao Y, Wu J, Hu Y, Chai Y, Song J, Duan J, Zhang S, Xu X
Cell reports 2023 Oct 31;42(10):113262

Implication of ferroptosis in hepatic toxicity upon single or combined exposure to polystyrene microplastics and cadmium.

Wang L, Zhang X, Xu M, Zheng G, Chen J, Li S, Cui J, Zhang S
Environmental pollution (Barking, Essex : 1987) 2023 Oct 1;334:122250

Eukaryotic translation initiation factor 2α kinase 2 in pancreatic cancer: An approach towards managing clinical prognosis and molecular immunological characterization.

Du HX, Wang H, Ma XP, Chen H, Dai AB, Zhu KX
Oncology letters 2023 Nov;26(5):478

Loss of ADAR1 in macrophages in combination with interferon gamma suppresses tumor growth by remodeling the tumor microenvironment.

Lin W, Luo Y, Wu J, Zhang H, Jin G, Guo C, Zhou H, Liang H, Xu X
Journal for immunotherapy of cancer 2023 Nov;11(11)

Cryptosporidium uses CSpV1 to activate host type I interferon and attenuate antiparasitic defenses.

Deng S, He W, Gong AY, Li M, Wang Y, Xia Z, Zhang XT, Huang Pacheco AS, Naqib A, Jenkins M, Swanson PC, Drescher KM, Strauss-Soukup JK, Belshan M, Chen XM
Nature communications 2023 Mar 16;14(1):1456

Dynamic mapping of proteome trafficking within and between living cells by TransitID.

Qin W, Cheah JS, Xu C, Messing J, Freibaum BD, Boeynaems S, Taylor JP, Udeshi ND, Carr SA, Ting AY
Cell 2023 Jul 20;186(15):3307-3324.e30

Dynamic mapping of proteome trafficking within and between living cells by TransitID.

Xu WQ, Cheah JS, Xu C, Messing J, Freibaum BD, Boeynaems S, Taylor JP, Udeshi ND, Carr SA, Ting AY
bioRxiv : the preprint server for biology 2023 Feb 8;

The collateral activity of RfxCas13d can induce lethality in a RfxCas13d knock-in mouse model.

Li Y, Xu J, Guo X, Li Z, Cao L, Liu S, Guo Y, Wang G, Luo Y, Zhang Z, Wei X, Zhao Y, Liu T, Wang X, Xia H, Kuang M, Guo Q, Li J, Chen L, Wang Y, Li Q, Wang F, Liu Q, You F
Genome biology 2023 Feb 1;24(1):20

The SARS-CoV-2 nucleocapsid protein suppresses innate immunity by remodeling stress granules to atypical foci.

He S, Gou H, Zhou Y, Wu C, Ren X, Wu X, Guan G, Jin B, Huang J, Jin Z, Zhao T
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 Dec;37(12):e23269

CCR4-NOT differentially controls host versus virus poly(a)-tail length and regulates HCMV infection.

Burgess HM, Grande R, Riccio S, Dinesh I, Winkler GS, Depledge DP, Mohr I
EMBO reports 2023 Dec 6;24(12):e56327

Microglial NLRP3 inflammasome activation mediates diabetes-induced depression-like behavior via triggering neuroinflammation.

Su WJ, Li JM, Zhang T, Cao ZY, Hu T, Zhong SY, Xu ZY, Gong H, Jiang CL
Progress in neuro-psychopharmacology & biological psychiatry 2023 Aug 30;126:110796

Salvianolic acid A regulates pyroptosis of endothelial cells via directly targeting PKM2 and ameliorates diabetic atherosclerosis.

Zhu J, Chen H, Le Y, Guo J, Liu Z, Dou X, Lu D
Frontiers in pharmacology 2022;13:1009229

The Amino Acid at Position 95 in the Matrix Protein of Rabies Virus Is Involved in Antiviral Stress Granule Formation in Infected Cells.

Kojima I, Onomoto K, Zuo W, Ozawa M, Okuya K, Naitou K, Izumi F, Okajima M, Fujiwara T, Ito N, Yoneyama M, Yamada K, Nishizono A, Sugiyama M, Fujita T, Masatani T
Journal of virology 2022 Sep 28;96(18):e0081022

LincRNA-EPS impairs host antiviral immunity by antagonizing viral RNA-PKR interaction.

Zhu J, Chen S, Sun LQ, Liu S, Bai X, Li D, Zhang F, Qiao Z, Li L, Yao H, Xia Y, Xu P, Jiang X, Chen Z, Yan Y, Ma F
EMBO reports 2022 May 4;23(5):e53937

Attenuation of Activated eIF2α Signaling by ISRIB Treatment After Spinal Cord Injury Improves Locomotor Function.

Chang L, Liu X, Chen J, Liu H, Wang G, Wang G, Liao X, Shen X
Journal of molecular neuroscience : MN 2022 Mar;72(3):585-597

Temporal control of the integrated stress response by a stochastic molecular switch.

Klein P, Kallenberger SM, Roth H, Roth K, Ly-Hartig TBN, Magg V, Aleš J, Talemi SR, Qiang Y, Wolf S, Oleksiuk O, Kurilov R, Di Ventura B, Bartenschlager R, Eils R, Rohr K, Hamprecht FA, Höfer T, Fackler OT, Stoecklin G, Ruggieri A
Science advances 2022 Mar 25;8(12):eabk2022

Bioinformatics analysis combined with experiments to verify potential autophagy genes in wound healing.

Wu YF, Fang DL, Wei J
Annals of translational medicine 2022 Jun;10(12):680

What goes around comes around: Artificial circular RNAs bypass cellular antiviral responses.

Breuer J, Barth P, Noe Y, Shalamova L, Goesmann A, Weber F, Rossbach O
Molecular therapy. Nucleic acids 2022 Jun 14;28:623-635

Viral evasion of PKR restriction by reprogramming cellular stress granules.

Gao P, Liu Y, Wang H, Chai Y, Weng W, Zhang Y, Zhou L, Ge X, Guo X, Han J, Yang H
Proceedings of the National Academy of Sciences of the United States of America 2022 Jul 19;119(29):e2201169119

The PERK/PKR-eIF2α Pathway Negatively Regulates Porcine Hemagglutinating Encephalomyelitis Virus Replication by Attenuating Global Protein Translation and Facilitating Stress Granule Formation.

Shi J, Li Z, Xu R, Zhang J, Zhou Q, Gao R, Lu H, Lan Y, Zhao K, He H, Gao F, He W
Journal of virology 2022 Jan 12;96(1):e0169521

Cardiovirus leader proteins retarget RSK kinases toward alternative substrates to perturb nucleocytoplasmic traffic.

Lizcano-Perret B, Lardinois C, Wavreil F, Hauchamps P, Herinckx G, Sorgeloos F, Vertommen D, Gatto L, Michiels T
PLoS pathogens 2022 Dec;18(12):e1011042

USP19 (ubiquitin specific peptidase 19) promotes TBK1 (TANK-binding kinase 1) degradation via chaperone-mediated autophagy.

Zhao X, Di Q, Yu J, Quan J, Xiao Y, Zhu H, Li H, Ling J, Chen W
Autophagy 2022 Apr;18(4):891-908

Maternal immune activation in mice disrupts proteostasis in the fetal brain.

Kalish BT, Kim E, Finander B, Duffy EE, Kim H, Gilman CK, Yim YS, Tong L, Kaufman RJ, Griffith EC, Choi GB, Greenberg ME, Huh JR
Nature neuroscience 2021 Feb;24(2):204-213

PKR activity modulation by phosphomimetic mutations of serine residues located three aminoacids upstream of double-stranded RNA binding motifs.

Cesaro T, Hayashi Y, Borghese F, Vertommen D, Wavreil F, Michiels T
Scientific reports 2021 Apr 28;11(1):9188

HDAC6, A Novel Cargo for Autophagic Clearance of Stress Granules, Mediates the Repression of the Type I Interferon Response During Coxsackievirus A16 Infection.

Zheng Y, Zhu G, Tang Y, Yan J, Han S, Yin J, Peng B, He X, Liu W
Frontiers in microbiology 2020;11:78

NSs amyloid formation is associated with the virulence of Rift Valley fever virus in mice.

Léger P, Nachman E, Richter K, Tamietti C, Koch J, Burk R, Kummer S, Xin Q, Stanifer M, Bouloy M, Boulant S, Kräusslich HG, Montagutelli X, Flamand M, Nussbaum-Krammer C, Lozach PY
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Mitoxantrone triggers immunogenic prostate cancer cell death via p53-dependent PERK expression.

Li C, Sun H, Wei W, Liu Q, Wang Y, Zhang Y, Lian F, Liu F, Li C, Ying K, Huo H, Qi Z, Li B
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Cellular & molecular immunology 2020 Apr;17(4):356-368

The Leader Protein of Theiler's Virus Prevents the Activation of PKR.

Borghese F, Sorgeloos F, Cesaro T, Michiels T
Journal of virology 2019 Oct 1;93(19)

Oxytocin Suppresses Inflammatory Responses Induced by Lipopolysaccharide through Inhibition of the eIF-2-ATF4 Pathway in Mouse Microglia.

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PKR knockout in the 5xFAD model of Alzheimer's disease reveals beneficial effects on spatial memory and brain lesions.

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p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.

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Burgess HM, Mohr I
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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: MCF7 cells were subjected to SDS PAGE followed by western blot with 18244-1-AP(EIF2AK2 antibody) at dilution of 1:2000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of MCF-7 cells, using EIF2AK2 antibody 18244-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human testis using 18244-1-AP(EIF2AK2 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human testis using 18244-1-AP(EIF2AK2 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue