18244-1-AP

antibody from Proteintech Group

Targeting: EIF2AK2 EIF2AK1, PKR, PPP1R83, PRKR

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

18244-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#18244-1-AP, RRID:AB_2246451

Product name

EIF2AK2, PKR antibody

Antibody type

Polyclonal

Description

KD/KO validated EIF2AK2, PKR antibody (Cat. #18244-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse , rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Attenuation of Activated eIF2α Signaling by ISRIB Treatment After Spinal Cord Injury Improves Locomotor Function.

Chang L, Liu X, Chen J, Liu H, Wang G, Wang G, Liao X, Shen X
Journal of molecular neuroscience : MN 2021 Oct 13;

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Maternal immune activation in mice disrupts proteostasis in the fetal brain.

Kalish BT, Kim E, Finander B, Duffy EE, Kim H, Gilman CK, Yim YS, Tong L, Kaufman RJ, Griffith EC, Choi GB, Greenberg ME, Huh JR
Nature neuroscience 2021 Feb;24(2):204-213

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USP19 (ubiquitin specific peptidase 19) promotes TBK1 (TANK-binding kinase 1) degradation via chaperone-mediated autophagy.

Zhao X, Di Q, Yu J, Quan J, Xiao Y, Zhu H, Li H, Ling J, Chen W
Autophagy 2021 Aug 26;:1-18

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PKR activity modulation by phosphomimetic mutations of serine residues located three aminoacids upstream of double-stranded RNA binding motifs.

Cesaro T, Hayashi Y, Borghese F, Vertommen D, Wavreil F, Michiels T
Scientific reports 2021 Apr 28;11(1):9188

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HDAC6, A Novel Cargo for Autophagic Clearance of Stress Granules, Mediates the Repression of the Type I Interferon Response During Coxsackievirus A16 Infection.

Zheng Y, Zhu G, Tang Y, Yan J, Han S, Yin J, Peng B, He X, Liu W
Frontiers in microbiology 2020;11:78

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NSs amyloid formation is associated with the virulence of Rift Valley fever virus in mice.

Léger P, Nachman E, Richter K, Tamietti C, Koch J, Burk R, Kummer S, Xin Q, Stanifer M, Bouloy M, Boulant S, Kräusslich HG, Montagutelli X, Flamand M, Nussbaum-Krammer C, Lozach PY
Nature communications 2020 Jul 1;11(1):3281

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Mitoxantrone triggers immunogenic prostate cancer cell death via p53-dependent PERK expression.

Li C, Sun H, Wei W, Liu Q, Wang Y, Zhang Y, Lian F, Liu F, Li C, Ying K, Huo H, Qi Z, Li B
Cellular oncology (Dordrecht) 2020 Dec;43(6):1099-1116

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ZBP1 mediates interferon-induced necroptosis.

Yang D, Liang Y, Zhao S, Ding Y, Zhuang Q, Shi Q, Ai T, Wu SQ, Han J
Cellular & molecular immunology 2020 Apr;17(4):356-368

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The Leader Protein of Theiler's Virus Prevents the Activation of PKR.

Borghese F, Sorgeloos F, Cesaro T, Michiels T
Journal of virology 2019 Oct 1;93(19)

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Oxytocin Suppresses Inflammatory Responses Induced by Lipopolysaccharide through Inhibition of the eIF-2-ATF4 Pathway in Mouse Microglia.

Inoue T, Yamakage H, Tanaka M, Kusakabe T, Shimatsu A, Satoh-Asahara N
Cells 2019 May 31;8(6)

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PKR knockout in the 5xFAD model of Alzheimer's disease reveals beneficial effects on spatial memory and brain lesions.

Tible M, Mouton Liger F, Schmitt J, Giralt A, Farid K, Thomasseau S, Gourmaud S, Paquet C, Rondi Reig L, Meurs E, Girault JA, Hugon J
Aging cell 2019 Jun;18(3):e12887

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p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.

Carugo A, Minelli R, Sapio L, Soeung M, Carbone F, Robinson FS, Tepper J, Chen Z, Lovisa S, Svelto M, Amin S, Srinivasan S, Del Poggetto E, Loponte S, Puca F, Dey P, Malouf GG, Su X, Li L, Lopez-Terrada D, Rakheja D, Lazar AJ, Netto GJ, Rao P, Sgambato A, Maitra A, Tripathi DN, Walker CL, Karam JA, Heffernan TP, Viale A, Roberts CWM, Msaouel P, Tannir NM, Draetta GF, Genovese G
Cancer cell 2019 Feb 11;35(2):204-220.e9

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Defining the Role of Stress Granules in Innate Immune Suppression by the Herpes Simplex Virus 1 Endoribonuclease VHS.

Burgess HM, Mohr I
Journal of virology 2018 Aug 1;92(15)

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Expression of Interferon Effector Gene SART1 Correlates with Interferon Treatment Response against Hepatitis B Infection.

Li Y, Zhu C, Wang F, Zhu T, Li J, Liu S, Xiao F
Mediators of inflammation 2016;2016:3894816

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Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.

Wu H, Wei L, Fan F, Ji S, Zhang S, Geng J, Hong L, Fan X, Chen Q, Tian J, Jiang M, Sun X, Jin C, Yin ZY, Liu Q, Zhang J, Qin F, Lin KH, Yu JS, Deng X, Wang HR, Zhao B, Johnson RL, Chen L, Zhou D
Nature communications 2015 Feb 19;6:6239

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 18244-1-AP(EIF2AK2 antibody) at dilution of 1:2000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using EIF2AK2 antibody 18244-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human testis using 18244-1-AP(EIF2AK2 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human testis using 18244-1-AP(EIF2AK2 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue