18244-1-AP

antibody from Proteintech Group

Targeting: EIF2AK2 EIF2AK1, PKR, PPP1R83, PRKR

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

18244-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#18244-1-AP, RRID:AB_2246451

Product name

EIF2AK2, PKR antibody

Antibody type

Polyclonal

Description

KD/KO validated EIF2AK2, PKR antibody (Cat. #18244-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse , rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

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International journal of antimicrobial agents 2024 May;63(5):107124

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African swine fever virus maintains de novo global cellular protein synthesis and inhibits stress granules formation via dephosphorylating eIF2α.

Gao H, Gao X, Liu X, Luo Y, Zhong J, Liu J, Yan L, Wang H, Gong L, Zhang G, Zheng Z, Sun Y
Veterinary microbiology 2024 Mar;290:109988

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Hyperoside inhibits EHV-8 infection via alleviating oxidative stress and IFN production through activating JNK/Keap1/Nrf2/HO-1 signaling pathways.

Wang T, Hu L, Li R, Ren H, Li S, Sun Q, Ding X, Li Y, Wang C, Li L
Journal of virology 2024 Apr 16;98(4):e0015924

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Human herpesvirus 8 ORF57 protein is able to reduce TDP-43 pathology: network analysis identifies interacting pathways.

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Human molecular genetics 2023 Oct 4;32(20):2966-2980

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Virus-induced lncRNA-BTX allows viral replication by regulating intracellular translocation of DHX9 and ILF3 to induce innate escape.

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Cell reports 2023 Oct 31;42(10):113262

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Oncology letters 2023 Nov;26(5):478

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Loss of ADAR1 in macrophages in combination with interferon gamma suppresses tumor growth by remodeling the tumor microenvironment.

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Journal for immunotherapy of cancer 2023 Nov;11(11)

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Cryptosporidium uses CSpV1 to activate host type I interferon and attenuate antiparasitic defenses.

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Nature communications 2023 Mar 16;14(1):1456

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Dynamic mapping of proteome trafficking within and between living cells by TransitID.

Qin W, Cheah JS, Xu C, Messing J, Freibaum BD, Boeynaems S, Taylor JP, Udeshi ND, Carr SA, Ting AY
Cell 2023 Jul 20;186(15):3307-3324.e30

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Dynamic mapping of proteome trafficking within and between living cells by TransitID.

Xu WQ, Cheah JS, Xu C, Messing J, Freibaum BD, Boeynaems S, Taylor JP, Udeshi ND, Carr SA, Ting AY
bioRxiv : the preprint server for biology 2023 Feb 8;

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The collateral activity of RfxCas13d can induce lethality in a RfxCas13d knock-in mouse model.

Li Y, Xu J, Guo X, Li Z, Cao L, Liu S, Guo Y, Wang G, Luo Y, Zhang Z, Wei X, Zhao Y, Liu T, Wang X, Xia H, Kuang M, Guo Q, Li J, Chen L, Wang Y, Li Q, Wang F, Liu Q, You F
Genome biology 2023 Feb 1;24(1):20

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The SARS-CoV-2 nucleocapsid protein suppresses innate immunity by remodeling stress granules to atypical foci.

He S, Gou H, Zhou Y, Wu C, Ren X, Wu X, Guan G, Jin B, Huang J, Jin Z, Zhao T
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 Dec;37(12):e23269

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CCR4-NOT differentially controls host versus virus poly(a)-tail length and regulates HCMV infection.

Burgess HM, Grande R, Riccio S, Dinesh I, Winkler GS, Depledge DP, Mohr I
EMBO reports 2023 Dec 6;24(12):e56327

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Microglial NLRP3 inflammasome activation mediates diabetes-induced depression-like behavior via triggering neuroinflammation.

Su WJ, Li JM, Zhang T, Cao ZY, Hu T, Zhong SY, Xu ZY, Gong H, Jiang CL
Progress in neuro-psychopharmacology & biological psychiatry 2023 Aug 30;126:110796

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Salvianolic acid A regulates pyroptosis of endothelial cells via directly targeting PKM2 and ameliorates diabetic atherosclerosis.

Zhu J, Chen H, Le Y, Guo J, Liu Z, Dou X, Lu D
Frontiers in pharmacology 2022;13:1009229

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The Amino Acid at Position 95 in the Matrix Protein of Rabies Virus Is Involved in Antiviral Stress Granule Formation in Infected Cells.

Kojima I, Onomoto K, Zuo W, Ozawa M, Okuya K, Naitou K, Izumi F, Okajima M, Fujiwara T, Ito N, Yoneyama M, Yamada K, Nishizono A, Sugiyama M, Fujita T, Masatani T
Journal of virology 2022 Sep 28;96(18):e0081022

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LincRNA-EPS impairs host antiviral immunity by antagonizing viral RNA-PKR interaction.

Zhu J, Chen S, Sun LQ, Liu S, Bai X, Li D, Zhang F, Qiao Z, Li L, Yao H, Xia Y, Xu P, Jiang X, Chen Z, Yan Y, Ma F
EMBO reports 2022 May 4;23(5):e53937

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Attenuation of Activated eIF2α Signaling by ISRIB Treatment After Spinal Cord Injury Improves Locomotor Function.

Chang L, Liu X, Chen J, Liu H, Wang G, Wang G, Liao X, Shen X
Journal of molecular neuroscience : MN 2022 Mar;72(3):585-597

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Temporal control of the integrated stress response by a stochastic molecular switch.

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Science advances 2022 Mar 25;8(12):eabk2022

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Bioinformatics analysis combined with experiments to verify potential autophagy genes in wound healing.

Wu YF, Fang DL, Wei J
Annals of translational medicine 2022 Jun;10(12):680

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What goes around comes around: Artificial circular RNAs bypass cellular antiviral responses.

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Molecular therapy. Nucleic acids 2022 Jun 14;28:623-635

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Viral evasion of PKR restriction by reprogramming cellular stress granules.

Gao P, Liu Y, Wang H, Chai Y, Weng W, Zhang Y, Zhou L, Ge X, Guo X, Han J, Yang H
Proceedings of the National Academy of Sciences of the United States of America 2022 Jul 19;119(29):e2201169119

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Cardiovirus leader proteins retarget RSK kinases toward alternative substrates to perturb nucleocytoplasmic traffic.

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USP19 (ubiquitin specific peptidase 19) promotes TBK1 (TANK-binding kinase 1) degradation via chaperone-mediated autophagy.

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Maternal immune activation in mice disrupts proteostasis in the fetal brain.

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Nature neuroscience 2021 Feb;24(2):204-213

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PKR activity modulation by phosphomimetic mutations of serine residues located three aminoacids upstream of double-stranded RNA binding motifs.

Cesaro T, Hayashi Y, Borghese F, Vertommen D, Wavreil F, Michiels T
Scientific reports 2021 Apr 28;11(1):9188

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HDAC6, A Novel Cargo for Autophagic Clearance of Stress Granules, Mediates the Repression of the Type I Interferon Response During Coxsackievirus A16 Infection.

Zheng Y, Zhu G, Tang Y, Yan J, Han S, Yin J, Peng B, He X, Liu W
Frontiers in microbiology 2020;11:78

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NSs amyloid formation is associated with the virulence of Rift Valley fever virus in mice.

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Nature communications 2020 Jul 1;11(1):3281

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Mitoxantrone triggers immunogenic prostate cancer cell death via p53-dependent PERK expression.

Li C, Sun H, Wei W, Liu Q, Wang Y, Zhang Y, Lian F, Liu F, Li C, Ying K, Huo H, Qi Z, Li B
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ZBP1 mediates interferon-induced necroptosis.

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Cellular & molecular immunology 2020 Apr;17(4):356-368

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The Leader Protein of Theiler's Virus Prevents the Activation of PKR.

Borghese F, Sorgeloos F, Cesaro T, Michiels T
Journal of virology 2019 Oct 1;93(19)

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Oxytocin Suppresses Inflammatory Responses Induced by Lipopolysaccharide through Inhibition of the eIF-2-ATF4 Pathway in Mouse Microglia.

Inoue T, Yamakage H, Tanaka M, Kusakabe T, Shimatsu A, Satoh-Asahara N
Cells 2019 May 31;8(6)

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PKR knockout in the 5xFAD model of Alzheimer's disease reveals beneficial effects on spatial memory and brain lesions.

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p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.

Carugo A, Minelli R, Sapio L, Soeung M, Carbone F, Robinson FS, Tepper J, Chen Z, Lovisa S, Svelto M, Amin S, Srinivasan S, Del Poggetto E, Loponte S, Puca F, Dey P, Malouf GG, Su X, Li L, Lopez-Terrada D, Rakheja D, Lazar AJ, Netto GJ, Rao P, Sgambato A, Maitra A, Tripathi DN, Walker CL, Karam JA, Heffernan TP, Viale A, Roberts CWM, Msaouel P, Tannir NM, Draetta GF, Genovese G
Cancer cell 2019 Feb 11;35(2):204-220.e9

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Defining the Role of Stress Granules in Innate Immune Suppression by the Herpes Simplex Virus 1 Endoribonuclease VHS.

Burgess HM, Mohr I
Journal of virology 2018 Aug 1;92(15)

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Expression of Interferon Effector Gene SART1 Correlates with Interferon Treatment Response against Hepatitis B Infection.

Li Y, Zhu C, Wang F, Zhu T, Li J, Liu S, Xiao F
Mediators of inflammation 2016;2016:3894816

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Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.

Wu H, Wei L, Fan F, Ji S, Zhang S, Geng J, Hong L, Fan X, Chen Q, Tian J, Jiang M, Sun X, Jin C, Yin ZY, Liu Q, Zhang J, Qin F, Lin KH, Yu JS, Deng X, Wang HR, Zhao B, Johnson RL, Chen L, Zhou D
Nature communications 2015 Feb 19;6:6239

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 18244-1-AP(EIF2AK2 antibody) at dilution of 1:2000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using EIF2AK2 antibody 18244-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human testis using 18244-1-AP(EIF2AK2 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human testis using 18244-1-AP(EIF2AK2 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue