Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00005610-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005610-M02, RRID:AB_828845
- Product name
- Mouse Monoclonal PKR Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. EIF2AK2 (1D11)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references PKR negatively regulates leukemia progression in association with PP2A activation, Bcl-2 inhibition and increased apoptosis.
PKR regulates proliferation, differentiation, and survival of murine hematopoietic stem/progenitor cells.
Increased expression of the dsRNA-activated protein kinase PKR in breast cancer promotes sensitivity to doxorubicin.
Cheng X, Bennett RL, Liu X, Byrne M, Stratford May W
Blood cancer journal 2013 Sep 6;3:e144
Blood cancer journal 2013 Sep 6;3:e144
PKR regulates proliferation, differentiation, and survival of murine hematopoietic stem/progenitor cells.
Liu X, Bennett RL, Cheng X, Byrne M, Reinhard MK, May WS Jr
Blood 2013 Apr 25;121(17):3364-74
Blood 2013 Apr 25;121(17):3364-74
Increased expression of the dsRNA-activated protein kinase PKR in breast cancer promotes sensitivity to doxorubicin.
Bennett RL, Carruthers AL, Hui T, Kerney KR, Liu X, May WS Jr
PloS one 2012;7(9):e46040
PloS one 2012;7(9):e46040
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PKR Antibody (1D11) [H00005610-M02] - Analysis of EIF2AK2 expression in transfected 293T cell line by EIF2AK2 monoclonal antibody (M02), clone 1D11.Lane 1: EIF2AK2 transfected lysate(62.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PKR Antibody (1D11) [H00005610-M02] - EIF2AK2 monoclonal antibody (M02), clone 1D11 Analysis of EIF2AK2 expression in K-562.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: PKR Antibody (1D11) [H00005610-M02] - Detection limit for recombinant GST tagged EIF2AK2 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PKR Antibody (1D11) [H00005610-M02] - Analysis of monoclonal antibody to EIF2AK2 on HeLa cell. Antibody concentration 30 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PKR Antibody (1D11) [H00005610-M02] - Analysis of monoclonal antibody to EIF2AK2 on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 1.5 ug/ml.
