PA5-20284

antibody from Invitrogen Antibodies

Targeting: EIF2AK2 EIF2AK1, PKR, PPP1R83, PRKR

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-20284

Provider

Invitrogen Antibodies

Product name

PKR Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is Caco-2 cell lysate.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Caco-2 whole cell lysate using a PKR polyclonal antibody (Product # PA5-20284) at (A) 1 and (B) 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation of PKR in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PKR Polyclonal Antibody (Product # PA5-20284) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation of PKR in MCF-7 Cells. Loading: 15 µg of lysates per lane. Antibodies: PKR Polyclonal Antibody (Product # PA5-20284) (A: 1 µg/mL; B: 2 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded rat lung tissue using PKR Polyclonal Antibody (Product # PA5-20284) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.