PA5-56292

antibody from Invitrogen Antibodies

Targeting: PLEC EBS1, PCN, PLEC1, PLTN

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-56292

Provider

Invitrogen Antibodies

Product name

Plectin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Immunogen sequence: TEIIRQQGLA SYDYVRRRLT AEDLFEARII SLETYNLLRE GTRSLREALE AESAWCYLYG TGSVAGVYLP GSRQTLSIYQ ALKKGLLSAE VARLLLEAQA A Highest antigen sequence identity to the following orthologs: Mouse - 90%, Rat - 92%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.3 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of Plectin in human cell line U-251 MG shows positivity in cytoplasm, intermediate filaments & focal adhesion sites. Samples were probed using a Plectin Polyclonal Antibody (Product # PA5-56292).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorecent analysis of Plectin in human cell line U-251 MG using Plectin Polyclonal Antibody (Product # PA5-56292). Staining shows localization to cytosol, intermediate filaments and focal adhesion sites.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Plectin was performed on U2OS cell lysates. Antibody-bead conjugates were prepared by adding 1 µg of PLEC polyclonal antibody (Product # PA5-56292) with 30 µL of protein A -Sepharose beads and rocked overnight at 4°C. 1 µg of PLEC KO lysate was incubated with antibody-bead conjugate for 2 hrs at 4°C. After multiple washes, 10% starting material (SM), 10% unbound fraction (UB) and immunoprecipitated fraction (IP) were processed for immunoblot using a PLEC monoclonal antibody. Ponceau stained transfer of blot is shown. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Plectin in human skeletal muscle using Plectin Polyclonal Antibody (Product # PA5-56292) shows high expression.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Plectin in human testis using Plectin Polyclonal Antibody (Product # PA5-56292).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Plectin in human liver, pancreas, skeletal muscle and testis using Plectin Polyclonal Antibody (Product # PA5-56292) (A) shows similar protein distribution across tissues to an independent Plectin Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Plectin in human liver using Plectin Polyclonal Antibody (Product # PA5-56292).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Plectin in human pancreas using Plectin Polyclonal Antibody (Product # PA5-56292) shows low expression as expected.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Plectin was performed on U2OS cell lysates. Antibody-bead conjugates were prepared by adding 1 µg of PLEC polyclonal antibody (Product # PA5-56292) with 30 µL of protein A -Sepharose beads and rocked overnight at 4°C. 1 µg of PLEC KO lysate was incubated with antibody-bead conjugate for 2 hrs at 4°C. After multiple washes, 10% starting material (SM), 10% unbound fraction (UB) and immunoprecipitated fraction (IP) were processed for immunoblot using a PLEC monoclonal antibody. Ponceau stained transfer of blot is shown. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.