13482-1-AP

antibody from Proteintech Group

Targeting: PPP2CA PP2AC, PP2Calpha

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

13482-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#13482-1-AP, RRID:AB_2169485

Product name

PPP2CA antibody

Antibody type

Polyclonal

Description

KD/KO validated PPP2CA antibody (Cat. #13482-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Phenotypic Analysis of Mice with Conditional Knockout of PPP2CA in Colonic Tissues.

Liu Z, Fang C, Li J
Digestive diseases and sciences 2026 Mar 3;

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Covalent targeting of PP2A scaffold subunit PPP2R1A by Pseudolarolide B enhances holoenzyme assembly to exert anti-inflammatory activity.

Li Y, Huang Y, Wang S, Xia H, Li J, Wu X, Lu Y, Ma B, Su G, Li Y
Bioorganic chemistry 2026 Jun 15;174:109684

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Analysis of GRK2 aggregation in the pathology of Alzheimer disease in animal models.

Abd Alla J, Perhal A, Fu X, Langer A, Faramawy YE, Quitterer U
Cell reports. Medicine 2026 Apr 21;7(4):102707

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LTF regulates glioblastoma progression and temozolomide resistance via the NF-κB signaling pathway.

Gao L, Xiao H, Lu H, Ma J, Zhu H
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2025;58:e13821

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PPP2CA knockdown upregulates the expression levels of ferroptosis-related genes TFRC and ACSL4 in colorectal cancer cells by promoting mTOR phosphorylation.

Cheng J, Liu Z, Liu H, Fang C, Li J
Frontiers in oncology 2025;15:1694932

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Canagliflozin inhibits hepatic stellate cells activation and proliferation by inhibiting PP2A-mediated β-catenin dephosphorylation to attenuate hepatic fibrosis.

Peng L, Zou Z, Zhou L, Li L, Lu W, Chen C, Xu Y, Liu Y, Lin J, Ye J
International immunopharmacology 2025 Oct 30;164:115347

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Obacunone ameliorates high-fat diet-induced MAFLD by regulating the PPARγ-FABP1/CD36 axis and the gut-liver crosstalk.

Wang X, Hong J, Liang R, Bao S, Li L, Wang J, Feng J
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Nov;147:157180

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Insight of action mechanism of Astragaloside IV for relieving of cerebral ischemic injury in a rat model of middle cerebral artery occlusion reperfusion via proteomics and network pharmacology.

Zhu XH, Yu X, Kong XW, Zhang Y, Jiang SL, Chai JH, Liang J, Kuang HX, Xia YG
Journal of natural medicines 2025 May;79(3):591-607

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STK39 inhibits antiviral immune response by inhibiting DCAF1-mediated PP2A degradation.

Zhang C, Xu P, Wang Y, Chen X, Pan Y, Ma Z, Wang C, Xu H, Zhou G, Zhu F, Xia H
Acta pharmaceutica Sinica. B 2025 Mar;15(3):1535-1551

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Colchicine inhibits vascular calcification by suppressing inflammasome activation through the enhancement of the Sirt2-PP2Ac signaling pathway.

Yang S, Huang H, Jiang K, Peng Y, Liang Z, Gong X, Li L, Li Y, Zhang B, Chen Y, Yang X
The Journal of biological chemistry 2025 Jul;301(7):110381

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T-cell differentiation stage block bias confers hypermethylation and mediastinal preference in T-cell lymphoblastic lymphoma.

Wang J, Qian B, Yu X, Zhang Y, Zhou C, Yang T, Xia L, Zhang G, Zhang YX, Wang Y, Fang Y
Clinical and translational medicine 2025 Jul;15(7):e70380

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PP2Acα regulates sleep amount and sleep homeostasis in mice.

Zhou S, Shi B, Chen L, Yin X, Liu M, Wu C, Wang H, Wang X, Li B, Wang F, Li Q, Liu Q
Communications biology 2025 Jul 8;8(1):1018

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IMPDH2 dephosphorylation under FGFR signaling promotes S-phase progression and tumor growth.

Zhou B, Zhao Q, Hou G, He J, Sha N, Zheng K, Peng H, Wang W, Zhou Y, Chen T, Jiang Y
Cell reports 2025 Jan 28;44(1):115116

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SMURF1 leads to the β-catenin signaling-mediated progression of esophageal squamous carcinoma by losing PATZ1-induced CCNG2 transcription.

Chen L, Tang J, Chang Y, Hang D, Ji J, Chen G
Biochemical pharmacology 2025 Feb;232:116688

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Acupuncture Modulates NMDAR-PP1/Calpain1-KCC2 Pathway to Ameliorate Spinal Hyperexcitability and Spastic Hemiplegia Induced by Ischemic Stroke.

He JL, Ma LX, Zhuang YX, Wen JS, Ma LH, Xiu JY, Chen MY
Journal of integrative neuroscience 2025 Dec 26;24(12):46980

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Carboxy-Methylation of the Catalytic Subunit of Protein Phosphatase 2A (PP2Ac) Integrates Methionine Availability with Methionine Addicted Cancer Cell Proliferation.

Andronicos A, Yoneda KC, Lin DW, Law FV, Bae H, Basirattalab A, Graham NA, Jang C, Kaiser P
Biomolecules 2025 Aug 22;15(9)

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gp78-regulated KAP1 phosphorylation induces radioresistance in breast cancer by facilitating PPP1CC/PPP2CA ubiquitination.

Han Y, Xiao M, Zhao S, Wang H, Li R, Xu B
iScience 2024 Sep 20;27(9):110847

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Exercise activates interferon response of the liver via Gpld1 to enhance antiviral innate immunity.

Ren T, He J, Zhang T, Niu A, Yuan Y, Zuo Y, Miao Y, Zhang H, Zang L, Qiao C, Cao X, Yang X, Zheng Z, Xu Y, Wu D, Zheng H
Science advances 2024 May 31;10(22):eadk5011

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Glycyrrhizic Acid Alleviates Semen Strychni-Induced Neurotoxicity Through the Inhibition of HMGB1 Phosphorylation and Inflammatory Responses.

Yu C, Xiang Y, Zhang M, Wen J, Duan X, Wang L, Deng G, Fang P
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2024 May 21;19(1):21

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Microplastics cause hepatotoxicity in diabetic mice by disrupting glucolipid metabolism via PP2A/AMPK/HNF4A and promoting fibrosis via the Wnt/β-catenin pathway.

Li X, Feng L, Kuang Q, Wang X, Yang J, Niu X, Gao L, Huang L, Luo P, Li L
Environmental toxicology 2024 Feb;39(2):1018-1030

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Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice.

Huang HZ, Ai WQ, Wei N, Zhu LS, Liu ZQ, Zhou CW, Deng MF, Zhang WT, Zhang JC, Yang CQ, Hu YZ, Han ZT, Zhang HH, Jia JJ, Wang J, Liu FF, Li K, Xu Q, Yuan M, Man H, Guo Z, Lu Y, Shu K, Zhu LQ, Liu D
Protein & cell 2024 Apr 1;15(4):261-284

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Berberine stimulates lysosomal AMPK independent of PEN2 and maintains cellular AMPK activity through inhibiting the dephosphorylation regulator UHRF1.

Ren G, Ding YW, Wang LL, Jiang JD
Frontiers in pharmacology 2023;14:1148611

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Hypercontractile cardiac phenotype in mice overexpressing the regulatory subunit PR72 of protein phosphatase 2A.

Herting JR, König JH, Hadova K, Heinick A, Müller FU, Pauls P, Seidl MD, Soppa C, Kirchhefer U
Frontiers in cardiovascular medicine 2023;10:1239555

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A mitochondrial EglN1-AMPKα axis drives breast cancer progression by enhancing metabolic adaptation to hypoxic stress.

Jiang W, Zhang M, Gao C, Yan C, Gao R, He Z, Wei X, Xiong J, Ruan Z, Yang Q, Li J, Li Q, Zhong Z, Zhang M, Yuan Q, Hu H, Wang S, Hu MM, Cai C, Wu GS, Jiang C, Zhang YL, Zhang CS, Zhang J
The EMBO journal 2023 Oct 16;42(20):e113743

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The CEP170B-KIF2A complex destabilizes microtubule minus ends to generate polarized microtubule network.

Guan C, Hua S, Jiang K
The EMBO journal 2023 Jun 1;42(11):e112953

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Classical swine fever virus NS5A protein activates autophagy via the PP2A-DAPK3-Beclin 1 axis.

Sun J, Yu H, Wang Y, Li L, Zhu J, Ma P, Feng Z, Tu C
Journal of virology 2023 Dec 21;97(12):e0098823

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GPx1 promotes hypertrophic differentiation of chondrocytes through modulation of akt signaling in a non-monotonic manner.

Mi S, Zhang J, Sun M, Huo X, Lv Y, Beier F, Lu S, Yan J
Experimental cell research 2023 Dec 15;433(2):113824

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Melatonin increases susceptibility to atrial fibrillation in obesity via Akt signaling impairment in response to lipid overload.

Qin X, Fu Y, Fan J, Liu B, Liu P, Zhang Y, Jiang T, Zheng Q
Journal of pineal research 2023 Apr;74(3):e12851

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Histone phosphorylation integrates the hepatic glucagon-PKA-CREB gluconeogenesis program in response to fasting.

Zhao Y, Li S, Chen Y, Wang Y, Wei Y, Zhou T, Zhang Y, Yang Y, Chen L, Liu Y, Hu C, Zhou B, Ding Q
Molecular cell 2023 Apr 6;83(7):1093-1108.e8

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Combination of berberine and low glucose inhibits gastric cancer through the PP2A/GSK3β/MCL-1 signaling pathway.

Peng Z, Wangmu T, Li L, Han G, Huang D, Yi P
European journal of pharmacology 2022 May 5;922:174918

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WSB1 regulates c-Myc expression through β-catenin signaling and forms a feedforward circuit.

Gao X, You J, Gong Y, Yuan M, Zhu H, Fang L, Zhu H, Ying M, He Q, Yang B, Cao J
Acta pharmaceutica Sinica. B 2022 Mar;12(3):1225-1239

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PP2A-dependent TFEB activation is blocked by PIKfyve-induced mTORC1 activity.

Hasegawa J, Tokuda E, Yao Y, Sasaki T, Inoki K, Weisman LS
Molecular biology of the cell 2022 Mar 1;33(3):ar26

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Berberine reduces hepatic ceramide levels to improve insulin resistance in HFD-fed mice by inhibiting HIF-2α.

Xia QS, Wu F, Wu WB, Dong H, Huang ZY, Xu L, Lu FE, Gong J
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2022 Jun;150:112955

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Nuclear UHRF1 is a gate-keeper of cellular AMPK activity and function.

Xu X, Ding G, Liu C, Ding Y, Chen X, Huang X, Zhang CS, Lu S, Zhang Y, Huang Y, Chen Z, Wei W, Liao L, Lin SH, Li J, Liu W, Li J, Lin SC, Ma X, Wong J
Cell research 2022 Jan;32(1):54-71

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Disruption of nuclear envelope integrity as a possible initiating event in tauopathies.

Prissette M, Fury W, Koss M, Racioppi C, Fedorova D, Dragileva E, Clarke G, Pohl T, Dugan J, Ahrens D, Chiu J, Hunt C, Siao CJ, Young T, Bhowmick A, Rogulin V, Desclaux M, Hayden EY, Podgorski M, Gao M, Macdonald LE, Frendewey D, Yancopoulos GD, Zambrowicz B
Cell reports 2022 Aug 23;40(8):111249

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Programmed cell death 10 promotes metastasis and epithelial-mesenchymal transition of hepatocellular carcinoma via PP2Ac-mediated YAP activation.

Sun B, Zhong FJ, Xu C, Li YM, Zhao YR, Cao MM, Yang LY
Cell death & disease 2021 Sep 14;12(9):849

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Sustained IKKβ phosphorylation and NF-κB activation by superoxide-induced peroxynitrite-mediated nitrotyrosine modification of B56γ3 and PP2A inactivation.

Yee YH, Chong SJF, Kong LR, Goh BC, Pervaiz S
Redox biology 2021 May;41:101834

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Vitamin D Attenuates Alzheimer-like Pathology Induced by Okadaic Acid.

Pan Y, Zhang Y, Liu N, Lu W, Yang J, Li Y, Liu Z, Wei Y, Lou Y, Kong J
ACS chemical neuroscience 2021 Apr 21;12(8):1343-1350

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BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells.

Chen B, Zhao Q, Xu T, Yu L, Zhuo L, Yang Y, Xu Y
Frontiers in cell and developmental biology 2020;8:774

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The proteasome activator REGγ accelerates cardiac hypertrophy by declining PP2Acα-SOD2 pathway.

Xie Y, Gao Y, Gao R, Yang W, Dong Z, Moses RE, Sun A, Li X, Ge J
Cell death and differentiation 2020 Oct;27(10):2952-2972

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Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.

Zheng H, Qi Y, Hu S, Cao X, Xu C, Yin Z, Chen X, Li Y, Liu W, Li J, Wang J, Wei G, Liang K, Chen FX, Xu Y
Science (New York, N.Y.) 2020 Nov 27;370(6520)

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Mouse 4T1 Breast Cancer Cell-Derived Exosomes Induce Proinflammatory Cytokine Production in Macrophages via miR-183.

Guo J, Duan Z, Zhang C, Wang W, He H, Liu Y, Wu P, Wang S, Song M, Chen H, Chen C, Si Q, Xiang R, Luo Y
Journal of immunology (Baltimore, Md. : 1950) 2020 Nov 15;205(10):2916-2925

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Protein phosphatase 1 catalyzes HBV core protein dephosphorylation and is co-packaged with viral pregenomic RNA into nucleocapsids.

Hu Z, Ban H, Zheng H, Liu M, Chang J, Guo JT
PLoS pathogens 2020 Jul;16(7):e1008669

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Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex.

He X, Li M, Yu H, Liu G, Wang N, Yin C, Tu Q, Narla G, Tao Y, Cheng S, Yin H
PLoS biology 2020 Dec;18(12):e3000803

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PP2Ac Modulates AMPK-Mediated Induction of Autophagy in Mycobacterium bovis-Infected Macrophages.

Hussain T, Zhao D, Shah SZA, Sabir N, Wang J, Liao Y, Song Y, Hussain Mangi M, Yao J, Dong H, Yang L, Zhou X
International journal of molecular sciences 2019 Nov 29;20(23)

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Role of type 2A phosphatase regulatory subunit B56α in regulating cardiac responses to β-adrenergic stimulation in vivo.

Puhl SL, Weeks KL, Güran A, Ranieri A, Boknik P, Kirchhefer U, Müller FU, Avkiran M
Cardiovascular research 2019 Mar 1;115(3):519-529

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KIAA1199 promotes metastasis of colorectal cancer cells via microtubule destabilization regulated by a PP2A/stathmin pathway.

Zhao L, Zhang D, Shen Q, Jin M, Lin Z, Ma H, Huang S, Zhou P, Wu G, Zhang T
Oncogene 2019 Feb;38(7):935-949

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MFHAS1 suppresses TLR4 signaling pathway via induction of PP2A C subunit cytoplasm translocation and inhibition of c-Jun dephosphorylation at Thr239.

Shi Q, Xiong B, Zhong J, Wang H, Ma D, Miao C
Molecular immunology 2017 Aug;88:79-88

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Glucocorticoid Receptor-mediated transactivation is hampered by Striatin-3, a novel interaction partner of the receptor.

Petta I, Bougarne N, Vandewalle J, Dejager L, Vandevyver S, Ballegeer M, Desmet S, Thommis J, De Cauwer L, Lievens S, Libert C, Tavernier J, De Bosscher K
Scientific reports 2017 Aug 21;7(1):8941

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Post-transcriptional modulation of protein phosphatase PPP2CA and tumor suppressor PTEN by endogenous siRNA cleaved from hairpin within PTEN mRNA 3'UTR in human liver cells.

Gao YE, Wang Y, Chen FQ, Feng JY, Yang G, Feng GX, Yang Z, Ye LH, Zhang XD
Acta pharmacologica Sinica 2016 Jul;37(7):898-907

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Cardiac function is regulated by B56α-mediated targeting of protein phosphatase 2A (PP2A) to contractile relevant substrates.

Kirchhefer U, Brekle C, Eskandar J, Isensee G, Kučerová D, Müller FU, Pinet F, Schulte JS, Seidl MD, Boknik P
The Journal of biological chemistry 2014 Dec 5;289(49):33862-73

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Reactivating PP2A by FTY720 as a novel therapy for AML with C-KIT tyrosine kinase domain mutation.

Yang Y, Huang Q, Lu Y, Li X, Huang S
Journal of cellular biochemistry 2012 Apr;113(4):1314-22

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human liver tissue were subjected to SDS PAGE followed by western blot with 13482-1-AP(PPP2CA antibody) at dilution of 1:300

Sample type

tissue

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP result of anti-PPP2CA (13482-1-AP for IP and Detection) with HeLa cells.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver cancer using 13482-1-AP(PPP2CA antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver cancer using 13482-1-AP(PPP2CA antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue