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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
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- Product number
- AF3989 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal PP2A alpha Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat PP2A when phosphorylated at Y307. The antigen is identical to the PP4 Y305 phosphorylation site.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Overcoming Tyrosine Kinase Inhibitor Resistance in Transformed Cell Harboring SEPT9-ABL1 Chimeric Fusion Protein.
The Prenylflavonoid Xanthohumol Reduces Alzheimer-Like Changes and Modulates Multiple Pathogenic Molecular Pathways in the Neuro2a/APPswe Cell Model of AD.
The Isoquinoline Alkaloid Dauricine Targets Multiple Molecular Pathways to Ameliorate Alzheimer-Like Pathological Changes In Vitro.
Sulfiredoxin-Peroxiredoxin IV axis promotes human lung cancer progression through modulation of specific phosphokinase signaling.
Kawai H, Matsushita H, Suzuki R, Kitamura Y, Ogawa Y, Kawada H, Ando K
Neoplasia (New York, N.Y.) 2019 Aug;21(8):788-801
Neoplasia (New York, N.Y.) 2019 Aug;21(8):788-801
The Prenylflavonoid Xanthohumol Reduces Alzheimer-Like Changes and Modulates Multiple Pathogenic Molecular Pathways in the Neuro2a/APPswe Cell Model of AD.
Huang X, Wang J, Chen X, Liu P, Wang S, Song F, Zhang Z, Zhu F, Huang X, Liu J, Song G, Spencer PS, Yang X
Frontiers in pharmacology 2018;9:199
Frontiers in pharmacology 2018;9:199
The Isoquinoline Alkaloid Dauricine Targets Multiple Molecular Pathways to Ameliorate Alzheimer-Like Pathological Changes In Vitro.
Liu P, Chen X, Zhou H, Wang L, Zhang Z, Ren X, Zhu F, Guo Y, Huang X, Liu J, Spencer PS, Yang X
Oxidative medicine and cellular longevity 2018;2018:2025914
Oxidative medicine and cellular longevity 2018;2018:2025914
Sulfiredoxin-Peroxiredoxin IV axis promotes human lung cancer progression through modulation of specific phosphokinase signaling.
Wei Q, Jiang H, Xiao Z, Baker A, Young MR, Veenstra TD, Colburn NH
Proceedings of the National Academy of Sciences of the United States of America 2011 Apr 26;108(17):7004-9
Proceedings of the National Academy of Sciences of the United States of America 2011 Apr 26;108(17):7004-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Phospho-PP2A (Y307), Catalytic Subunit by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with hydroxyurea. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3989), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PP2A (Y307), Catalytic Subunit at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
