Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN497954 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (AA 1-231), (N-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 231 of PP2A
- Description
- Antigen-Affinity Chromatography.
- Reactivity
- Human, Rat, Bovine, Canine, Rabbit
- Host
- Rabbit
- Epitope
- AA 1-231,N-Term
- Vial size
- 0.1 mL
- Concentration
- 0.52 mg/mL
- Storage
- Store the antibody undiluted (in aliquots) at -20°C.
- Handling
- Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western Blot analysis: AP19055PU-N PPP2CA antibody staining of Molt-4 whole cell lysate (30 μg) at 1/1000 dilution.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western Blot analysis: AP19055PU-N PPP2CA antibody staining of Mouse brain cell lysate (50 μg) at 1/1000 dilution. 10% SDS PAGE
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using AP19055PU-N PPP2CA antibody (Green) at 1/500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1/2000.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunohistochemistry: AP19055PU-N PPP2CA antibody staining of Paraffin-Embedded Cal27 xenograft at 1/100 dilution.