PA5-17980

antibody from Invitrogen Antibodies

Targeting: PPP2CB PP2Abeta

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-17980

Provider

Invitrogen Antibodies

Product name

PP2A alpha/beta Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, canine, mouse, porcine, rabbit and rat based on sequence homology.

Reactivity

Human, Mouse, Rat, Porcine

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PP2A alpha/beta by a PP2A alpha/beta monoclonal antibody (Product # PA5-17980) at a concentration of 0.1 µg/mL. Mouse (A), Rat (B) and Pig (C) Brain lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot staining of Human Cerebellum lysate using Product # PA5-17980 at a concentration of 0.05 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts of PC-3 (Lane 1), HEL 92.1.7 (Lane 2), K-562 (Lane 3), A549 (Lane 4), Jurkat (Lane 5) and A-431 (Lane 6). The blot was probed with Anti-PP2A alpha/beta Polyclonal Antibody (Product # PA5-17980, 2 µg/ml) and detected by chemiluminescence using Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27014, 0.25 µg/ml, 1:4000 dilution). A 36 kDa band corresponding to PP2A alpha/beta was observed across the cell lines tested. A regulatory subunit band around 65 kDa was observed across the cell lines tested.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PP2A alpha/beta was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PP2A alpha/beta Polyclonal Antibody (Product # PA5-17980) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit-anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A-11078) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of PP2A alpha beta in paraffin embedded human kidney using a PP2A alpha beta polyclonal antibody (Product #PA5-17980) at a concentration of 10 µg/mL. Steamed antigen retrieval was performed with pH 6 buffered citrate. Samples were then stained with alkaline phosphatase.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of PP2A alpha beta in paraffin embedded human kidney using a PP2A alpha beta polyclonal antibody (Product #PA5-17980) at a concentration of 10 µg/mL. Steamed antigen retrieval was performed with pH 6 buffered citrate. Samples were then stained with alkaline phosphatase.