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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NB100-2260 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2260, RRID:AB_10003478
- Product name
- Rabbit Polyclonal PP2A alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Regulation of Greatwall kinase during Xenopus oocyte maturation.
Yamamoto TM, Blake-Hodek K, Williams BC, Lewellyn AL, Goldberg ML, Maller JL
Molecular biology of the cell 2011 Jul 1;22(13):2157-64
Molecular biology of the cell 2011 Jul 1;22(13):2157-64
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PP2A alpha Antibody [NB100-2260] - Detection of Human and Mouse PP2A by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody NB100-2260 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PP2A alpha Antibody [NB100-2260] - Detection of Human and Mouse PP2A on HeLa whole cell lysate using NB100-2260.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PP2A alpha Antibody [NB100-2260] - Detection of human PP2A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody NB100-2260 (lot 2) used for IP at 6 ug per reaction. PP2A was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-PP2A antibody NB100-2259. For blotting immunoprecipitated PP2A, NB100-2260 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
