Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00002185-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002185-M01, RRID:AB_547452
- Product name
- Mouse Monoclonal PYK2/FAK2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PTK2B - PTK2B protein tyrosine kinase 2 beta
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PYK2/FAK2 Antibody (1F9) [H00002185-M01] - Analysis of PTK2B expression in transfected 293T cell line by PTK2B monoclonal antibody (M01), clone 1F9.Lane 1: PTK2B transfected lysate(115.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PYK2/FAK2 Antibody (1F9) [H00002185-M01] - PTK2B monoclonal antibody (M01), clone 1F9 Analysis of PTK2B expression in K-562.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: PYK2/FAK2 Antibody (1F9) [H00002185-M01] - Detection limit for recombinant GST tagged PTK2B is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PYK2/FAK2 Antibody (1F9) [H00002185-M01] - Analysis of PTK2B transfected lysate using anti-PTK2B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PTK2B MaxPab rabbit polyclonal antibody.