Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-C154006 - Provider product page
- Provider
- LSBio
- Product name
- STAT5A Antibody (phospho-Tyr694, clone 5F6.F1) LS-C154006
- Antibody type
- Monoclonal
- Description
- Protein A affinity chromatography
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5F6.F1
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-Stat5 pY694 Monoclonal Antibody - Western Blot. Western blot of Protein A purified Mouse Monoclonal anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kD) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1000 dilution, overnight at 4C. For detection DyLight800 conjugated Gt-a-Mouse IgG was used at a 1:20000 dilution for 30 min at room temperature followed by visualization using a VersaDoc MP 4000 imaging system (Bio-Rad).
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-Stat5 pY694 Monoclonal Antibody - Immunohistochemistry. Immunohistochemistry using Anti-Stat5 pY694 monoclonal antibody shows detection of phosphorylated Stat5pY694 in human breast tissue (40X). The antibody was used a dilution to 20 ug/mL. The image shows breast epithelium with moderate nuclear staining. Tissue was formalin fixed and paraffin embedded. Antigen retrieval: steam sections in 0.1 M sodium citrate buffer, pH 6, for 20 min. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.