MA5-17183

antibody from Invitrogen Antibodies

Targeting: STAT5A MGF, STAT5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-17183

Provider

Invitrogen Antibodies

Product name

STAT5 alpha Monoclonal Antibody (6D4)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-17183 targets STAT5A in FACS, ICC, IHC, and WB applications and shows reactivity with Human samples. The MA5-17183 immunogen is purified recombinant fragment of human STAT5A (amino acids: 583-794 ) expressed in E. Coli. MA5-17183 detects STAT5A which has a predicted molecular weight of approximately 90.6kDa.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

6D4

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of STAT5A using a STAT5A monoclonal antibody (Product # MA5-17183) against a human STAT5A recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of STAT5A using STAT5A monoclonal antibody (Product # MA5-17183) in K562 (1), MOLT4 (2), HeLa (3), Jurkat (4), and A431 (5) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of STAT5A using a STAT5A monoclonal antibody (Product # MA5-17183) against a human STAT5A recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of STAT5A using STAT5A monoclonal antibody (Product # MA5-17183) in K562 (1), MOLT4 (2), HeLa (3), Jurkat (4), and A431 (5) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using STAT5A monoclonal antibody (Product # MA5-17183) (Green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using STAT5A monoclonal antibody (Product # MA5-17183) followed with DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using STAT5A monoclonal antibody (Product # MA5-17183) followed with DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of K562 cells using STAT5A monoclonal antibody (Product # MA5-17183) (green) and negative control (red).