Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB41901 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal STAT5a/b Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human STAT5a/b when phosphorylated at Y964/Y699 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Phospho-STAT5a/b (Y694/Y699) by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-alpha A (Catalog # 11100-1) for 20 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-STAT5a/b (Y694/Y699) Monoclonal Antibody (Catalog # MAB41901) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STAT5a/b at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Phospho-STAT5a/b (Y694/Y699) in IFN alpha-treated Daudi Human Cell Line by Flow Cytometry. Detection of Phospho-STAT5a/b (Y694/Y699) in IFN alpha-treated Daudi Human Cell Line by Flow Cytometry.