699603

antibody from BioLegend

Targeting: STAT5A MGF, STAT5

Flow cytometry (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

699603

Provider

BioLegend

Proper citation

BioLegend Cat#699603, RRID:AB_2734545

Product name

Go-ChIP-Grade™ Purified anti-STAT5 Phospho (Tyr694)

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Mouse

Antibody clone number

A17016B

Vial size

25 µg

Concentration

0.50 mg/mL

Storage

2°C-8°C

Handling

Ambient RT

Chromatin Immunoprecipitation

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 x 10⁶ of Ba/F3 cells deprived for IL-3 overnight and then treated with IL-3 with either A)1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT5 Phospho (Tyr694) (Clone A17016B) or B) equal amount of Purified Mouse IgG1, ? Isotype Control Antibody (Clone MG1-45, Cat. No. 401401) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting mouse SOCS3 gene region and ?-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.

Conjugate

Unconjugated