Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- 699604 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#699604, RRID:AB_2734546
- Product name
- Go-ChIP-Grade™ Purified anti-STAT5 Phospho (Tyr694)
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
- Antibody clone number
- A17016B
- Vial size
- 100 µg
- Concentration
- 0.50 mg/mL
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 x 106 of Ba/F3 cells deprived for IL-3 overnight and then treated with IL-3 with either A)1:50 dilution of Go-ChIP-Grade Purified anti-STAT5 Phospho (Tyr694) (Clone A17016B) or B) equal amount of Purified Mouse IgG1, ? Isotype Control Antibody (Clone MG1-45, Cat. No. 401401) by using Go-ChIP-Grade Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting mouse SOCS3 gene region and ?-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
- Conjugate
- Unconjugated