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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-77817 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77817, RRID:AB_11023577
- Product name
- Mouse Monoclonal STAT5A Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. This antibody is specific for mouse Stat5a protein phosphorylated at Y694. A BLAST analysis was used to suggest cross-reactivity with Stat5a from human, mouse.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STAT5a [p Tyr694] Antibody (5F6.F1) [NBP1-77817] - Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution, overnight at 4C. For detection Dye light 800 conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 30 min at room temperature
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: STAT5a [p Tyr694] Antibody (5F6.F1) [NBP1-77817] - Analysis of phosphorylated Stat5pY694 in human breast tissue (40X). The antibody was used at a dilution of 1 to 20 ug/mL. The image shows breast epithelium with moderate nuclear staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
