MAB1458

antibody from Novus Biologicals

Targeting: HTRA2 OMI, PARK13, PRSS25

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

MAB1458

Provider

Novus Biologicals

Product name

Mouse Monoclonal HTRA2/Omi Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects the mitochondria-processed form of human HTRA2/Omi.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

TNF provokes cardiomyocyte apoptosis and cardiac remodeling through activation of multiple cell death pathways.

Haudek SB, Taffet GE, Schneider MD, Mann DL
The Journal of clinical investigation 2007 Sep;117(9):2692-701

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Cytosolic factor- and TOM-independent import of C-tail-anchored mitochondrial outer membrane proteins.

Setoguchi K, Otera H, Mihara K
The EMBO journal 2006 Dec 13;25(24):5635-47

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human HTRA2/Omi by Western Blot. Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line, MCF-7 human breast cancer cell line, HEK293 human embryonic kidney cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human HTRA2/Omi Monoclonal Antibody (Catalog # MAB1458) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HTRA2/Omi at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.