MAB0099

antibody from Abnova Corporation

Targeting: HTRA2 OMI, PARK13, PRSS25

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB0099

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB0099, RRID:AB_1019020

Product name

HTRA2 monoclonal antibody, clone 196C429

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against synthetic peptide of HTRA2.

Isotype

IgG

Antibody clone number

196C429

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

X-linked inhibitor of apoptosis (XIAP) protein protects against caspase activation and tissue loss after neonatal hypoxia-ischemia.

Wang X, Zhu C, Wang X, Hagberg H, Korhonen L, Sandberg M, Lindholm D, Blomgren K
Neurobiology of disease 2004 Jun;16(1):179-89

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Immunohistochemical analysis of Omi/HtrA2 expression in stomach cancer.

Lee SH, Lee JW, Kim HS, Kim SY, Park WS, Kim SH, Lee JY, Yoo NJ
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2003 May;111(5):586-90

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot detection of HTRA2 in HL-60 cell lysate. Two protein bands of approximate molecular weight of 50 and 38 KDa were detected using HTRA2 monoclonal antibody, clone 196C429 (Cat # MAB0099) at 3 ug/mL dilution. The 50 KDa and 38 KDa bands may represent precursor and mature form of Omi.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

IHC testing using HTRA2 monoclonal antibody, clone 196C429 (Cat # MAB0099) shows cytoplasmic staining in stomach tumor tissue (A) and very weak staining in normal stomach tissue (B) .

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)