LS-C499090

antibody from LSBio

Targeting: HTRA2 OMI, PARK13, PRSS25

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C499090

Provider

LSBio

Product name

HTRA2 / OMI Antibody LS-C499090

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Positive WB detected in:HEK293 whole cell lysate,Jurkat whole cell lysate,MCF-7 whole cell lysate,Raji whole cell lysate;All lanes:HTRA2 antibody at 3?g/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 49,39,47,40 KDa;Observed band size: 49 KDa;

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating GARS in MCF-7 whole cell lysate;Lane 1: Rabbit monoclonal IgG(1?g)instead of HTRA2 Antibody in MCF-7 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000);Lane 2: HTRA2 Antibody(8?g)+ MCF-7 whole cell lysate(500?g);Lane 3: MCF-7 whole cell lysate (20?g);

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating HTRA2 in MCF-7 whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of HTRA2 Antibody in MCF-7 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: HTRA2 Antibody (8µg) + MCF-7 whole cell lysate (500µg) Lane 3: MCF-7 whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating GARS in MCF-7 whole cell lysate;Lane 1: Rabbit monoclonal IgG(1?g)instead of HTRA2 Antibody in MCF-7 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000);Lane 2: HTRA2 Antibody(8?g)+ MCF-7 whole cell lysate(500?g);Lane 3: MCF-7 whole cell lysate (20?g);

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating HTRA2 in MCF-7 whole cell lysate Lane 1: Rabbit monoclonal IgG (1µg) instead of HTRA2 Antibody in MCF-7 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: HTRA2 Antibody (8µg) + MCF-7 whole cell lysate (500µg) Lane 3: MCF-7 whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating GARS in MCF-7 whole cell lysate;Lane 1: Rabbit monoclonal IgG(1?g)instead of HTRA2 Antibody in MCF-7 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000);Lane 2: HTRA2 Antibody(8?g)+ MCF-7 whole cell lysate(500?g);Lane 3: MCF-7 whole cell lysate (20?g);

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue using HTRA2 Antibody at dilution of 1:100

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using HTRA2 Antibody at dilution of 1:100