Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- M01941 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-HTRA2/Omi Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for Omi/HTRA2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IP, IHC, WB. Reactive species: Human, Mouse, Rat Omi/HTRA2 information: Molecular Weight: 48841 MW; Subcellular Localization: Mitochondrion intermembrane space. Mitochondrion membrane ; Single-pass membrane protein . Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID; Tissue Specificity: Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antibody clone number
- EEH-8
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
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Supportive validation
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- Boster Biological Technology (provider)
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- Experimental details
- Western blot analysis of HTRA2 expression in Jurkat cell lysate (M01941). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTRA2 monoclonal antibody (Catalog # M01941) overnight at 4
- Additional image