Antibody data
- Antibody Data
 - Antigen structure
 - References [0]
 - Comments [0]
 - Validations
 - Western blot [7]
 - Immunohistochemistry [8]
 - Other assay [1]
 
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Validation data
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- Product number
 - 15775-1-AP - Provider product page

 - Provider
 - Invitrogen Antibodies
 - Product name
 - HTRA2 Polyclonal Antibody
 - Antibody type
 - Polyclonal
 - Antigen
 - Other
 - Reactivity
 - Human, Mouse, Rat
 - Host
 - Rabbit
 - Isotype
 - IgG
 - Vial size
 - 150 µL
 - Concentration
 - 0.19 mg/mL
 - Storage
 - -20°C
 
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					Supportive validation
					
									
				
				- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Jurkat cells were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - HEK-293 cells were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - MCF7 cells were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - COS-7 cells were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - HeLa cells were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Raji cells were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
 
							
					Supportive validation
					
									
				
				- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 10x lens).
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 40x lens).
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry of paraffin-embedded human thyroid tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 10x lens).
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry of paraffin-embedded human thyroid tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 40x lens).
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 10x lens).
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 40x lens).
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry of paraffin-embedded human kidney using 15775-1-AP (HTRA2 antibody) at dilution of 1:100 (under 10x lens).
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry of paraffin-embedded human kidney using 15775-1-AP (HTRA2 antibody) at dilution of 1:100 (under 40x lens).
 
							
					Supportive validation
					
									
				
		- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - IP result of anti-HTRA2 (IP:15775-1-AP, 4ug; Detection:15775-1-AP 1:500) with Raji cells lysate 2000ug.