Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA1-41040 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HTRA2 Monoclonal Antibody (196C429)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: HL-60 or Jurkat whole cell lysate.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 196C429
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot detection of HtrA2 using a HtrA2 monoclonal antibody (Product # MA1-41040). HL-60 cell lysate was probed with a HtrA2 monoclonal antibody (Product # MA1-41040) at 3 µg/mL and two protein bands of approximate molecular weight of 50 and 38 kDa were detected. The 50 kDa and 38 kDa bands may represent precursor and mature forms of HtrA2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HTRA2 in HL-60 cell lysate. Samples were incubated in HTRA2 monoclonal antibody (Product # MA1-41040 using a dilution of 3 µg/mL. Two protein bands of approximate molecular weight of 50 and 38 kDa were detected. The 50 kDa and 38 kDa bands may represent precursor and mature forms of HtrA2.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HTRA2 in tumor and normal stomach tissue. Samples were incubated in HTRA2 monoclonal antibody (Product # MA1-41040). Stomach tumor tissue (A) and very weak staining in normal stomach tissue (B).