Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
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Validation data
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- Product number
- SM6012S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#SM6012S, RRID:AB_1617383
- Product name
- anti HTRA2 / PRSS25
- Antibody type
- Monoclonal
- Antigen
- Recombinant human HtrA2/Omi (134-458 aa) purified from E. coli
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1B3
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis: The extracts of 293T, MCF7, HT-29, HL-60, HeLa, mouse brain, and rat brain were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HtrA2/Omi antibody (1:1,000). Proteins werevisualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- The extracts of 293T, MCF7, HT-29, HL-60, HeLa, mouse brain, and rat brain were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HtrA2/Omi antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.