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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AM20292AF-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM20292AF-N, RRID:AB_10755809
- Product name
- anti HTRA2 / PRSS25
- Antibody type
- Monoclonal
- Antigen
- Human recombinant 6His tagged HtrA2/Omi.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 18-1-83
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of HtrA2/Omi expression in Human placenta (Lane 1), THP-1 cells (Lane 2), 293T cells (Lane 3), MCF7 cells (Lane 4), HeLa cells (Lane 5) and SK-N-SH cells (Lane 6) using AM20292AF-N HtrA2 antibody.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunoprecipitation of HtrA2/Omi from HeLa cells with AM20292AF-N 1 µg (Lane 1), 10 µg (Lane 2) or Mouse IgG2a (Lane 3). After immunoprecipitation with the antibody, immunocomplex was resolved on SDS- PAGE and immunoblotted with AM20292AF-N.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemical detection of HtrA2/ Omi on Paraffin Embedded Section of breast cancer with AM20292AF-N HtrA2 antibody.
