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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- MAB1458 - Provider product page
- Provider
- R&D Systems
- Product name
- Human HTRA2/Omi Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects the mitochondria-processed form of human HTRA2/Omi.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O43464- Isotype
- IgG
- Antibody clone number
- 229926
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references TNF provokes cardiomyocyte apoptosis and cardiac remodeling through activation of multiple cell death pathways.
Cytosolic factor- and TOM-independent import of C-tail-anchored mitochondrial outer membrane proteins.
Haudek SB, Taffet GE, Schneider MD, Mann DL
The Journal of clinical investigation 2007 Sep;117(9):2692-701
The Journal of clinical investigation 2007 Sep;117(9):2692-701
Cytosolic factor- and TOM-independent import of C-tail-anchored mitochondrial outer membrane proteins.
Setoguchi K, Otera H, Mihara K
The EMBO journal 2006 Dec 13;25(24):5635-47
The EMBO journal 2006 Dec 13;25(24):5635-47
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human HTRA2/Omi by Western Blot. Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line, MCF-7 human breast cancer cell line, HEK293 human embryonic kidney cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human HTRA2/Omi Monoclonal Antibody (Catalog # MAB1458) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HTRA2/Omi at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
