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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX61061 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX61061, RRID:AB_10623754
- Product name
- PKA R2 (phospho Ser99) antibody [E151]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Rabbit
- Storage
- Shipped at 4¢XC. Store at +4¢XC short term (1-2 weeks). Upon delivery aliquot. Store at -20¢XC. Avoid freeze / thaw cycle.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- C. Western blot analysis on K562 cell lysate using anti-Phospho-PKA RII (pS96) RabMAb (cat. #GTX61061) 1:2500 dilution. Cell lysates were either (1) untreated or (2) treated with Alkaline Phosphatase.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of (1) untreated and (2) Alkaline Phosphatase-treated K562 lysates using PKA R2 (phospho Ser99) antibody [E151] at a dilution of 1:2500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of (A) untreated and (B) Alkaline Phosphatase treated HeLa cells using PKA R2 (phospho Ser99) antibody [E151] at a dilution of 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of (A) untreated and (B) Phosphatase treated lymph node metastatic tissues using PKA R2 (phospho Ser99) antibody [E151] at a dilution of 1:250.
