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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- CF501106 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRKAR2A Monoclonal Antibody (OTI1C4), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI1C4
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Equivalent amounts of cell lysates (10 µg per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and PRKAR2A-Knockout HeLa cells (KO, Cat# LC812853) were separated by SDS-PAGE and immunoblotted with anti-PRKAR2A monoclonal antibody TA501106(1:2000). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR2A (RC220376, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKA. Positive lysates LY401337 (100 µg) and LC401337 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Equivalent amounts of cell lysates (10 µg per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and PRKAR2A-Knockout HeLa cells (KO, Cat# LC812853) were separated by SDS-PAGE and immunoblotted with anti-PRKAR2A monoclonal antibody TA501106(1:2000). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-PRKAR2A mouse monoclonal antibody (TA501106) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKAR2A(RC220376).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PRKAR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501106, Dilution 1:50)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-PRKAR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501106, Dilution 1:50)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC220376 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKAR2A antibody (TA501106), and then analyzed by flow cytometry.
