Antibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- MA3-517 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-PRKAR2A Monoclonal Antibody (H2 006 D4)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA3-600 detects caveolin from human, rat and hamster samples. MA3-600 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody detects a 19-21 kDa band from transfected HEK293 (B8) cells, with a non-specific band appearing at 65-70 kDa. The MA3-600 immunogen is intercellular membrane protein-containing vesicles obtained from rat.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- H2 006 D4
- Vial size
- 100 µL
- Concentration
- Lot Dependent
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Adenylyl cyclase 3/adenylyl cyclase-associated protein 1 (CAP1) complex mediates the anti-migratory effect of forskolin in pancreatic cancer cells.
Quinn SN, Graves SH, Dains-McGahee C, Friedman EM, Hassan H, Witkowski P, Sabbatini ME
Molecular carcinogenesis 2017 Apr;56(4):1344-1360
Molecular carcinogenesis 2017 Apr;56(4):1344-1360
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Chromatin immunoprecipitation analysis of PKARII was performed using cross-linked chromatin from Human 5A8 J-lat T lymphocytes culture treated with 10 µg/mL PHA (phytohemaglutin) for 0, 2, and 24 hours. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0ul/100ul well volume of a PKAR11 monoclonal antibody (Product # MA3-517). Chromatin aliquots from ~10,000 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers shown to amplify -15kb and exon-1 of the EGR1 gene. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. Schematic representations of the EGR-1 locus are shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by the primers are represented by black bars. Data courtesy of Dr. Karol Bomsztyk at the University of Washington, Seattle, WA.