MA3-517

antibody from Invitrogen Antibodies

Targeting: PRKAR2A PRKAR2

Western blot (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

MA3-517

Provider

Invitrogen Antibodies

Product name

Anti-PRKAR2A Monoclonal Antibody (H2 006 D4)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA3-600 detects caveolin from human, rat and hamster samples. MA3-600 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody detects a 19-21 kDa band from transfected HEK293 (B8) cells, with a non-specific band appearing at 65-70 kDa. The MA3-600 immunogen is intercellular membrane protein-containing vesicles obtained from rat.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

H2 006 D4

Vial size

100 µL

Concentration

Lot Dependent

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Adenylyl cyclase 3/adenylyl cyclase-associated protein 1 (CAP1) complex mediates the anti-migratory effect of forskolin in pancreatic cancer cells.

Quinn SN, Graves SH, Dains-McGahee C, Friedman EM, Hassan H, Witkowski P, Sabbatini ME
Molecular carcinogenesis 2017 Apr;56(4):1344-1360

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin immunoprecipitation analysis of PKARII was performed using cross-linked chromatin from Human 5A8 J-lat T lymphocytes culture treated with 10 µg/mL PHA (phytohemaglutin) for 0, 2, and 24 hours. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0ul/100ul well volume of a PKAR11 monoclonal antibody (Product # MA3-517). Chromatin aliquots from ~10,000 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers shown to amplify -15kb and exon-1 of the EGR1 gene. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. Schematic representations of the EGR-1 locus are shown above the data where boxes represent exons (black boxes = translated regions, white boxes = untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by the primers are represented by black bars. Data courtesy of Dr. Karol Bomsztyk at the University of Washington, Seattle, WA.