TA321299

antibody from OriGene

Targeting: BAK1 BAK, BCL2L7, CDN1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA321299

Provider

OriGene

Product name

Anti-BAK1 rabbit polyclonal antibody

Antibody type

Polyclonal

Description

Anti-BAK1 rabbit polyclonal antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

BAK1

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

1.8mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 23kDa. Positive control: Hela, A431, 293T and Jurkat cell lysate. Recommended dilution: 1/1000-5000. (Gel: 12%SDS-PAGE Lane1-4: Hela, A431, 293T and Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/900 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 5 seconds)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using BAK1 antibody at dilution 1/50, on the right is treated with the fusion protein. (Original magnification:x200)

Validation comment

IHC