Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-27093 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BAK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BAK1 using 30 µg of A) HeLa (B) HepG2 and C) MOLT4 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a BAK1 polyclonal antibody (Product # PA5-27093) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using BAK Polyclonal Antibody (Product # PA5-27093). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with BAK Polyclonal Antibody (Product # PA5-27093) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human liver, bile duct, using BAK1 (Product # PA5-27093) antibody (10 µg/mL). Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.