Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Western blot [8]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX107678 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX107678, RRID:AB_1951681
- Product name
- NFkB p65 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references A high-throughput pipeline for validation of antibodies.
Monoacylglycerol lipase regulates cannabinoid receptor 2-dependent macrophage activation and cancer progression.
Acetylation state of RelA modulated by epigenetic drugs prolongs survival and induces a neuroprotective effect on ALS murine model.
2,3,5,4'-Tetrahydroxystilbene-2-O-β-glucoside Isolated from Polygoni Multiflori Ameliorates the Development of Periodontitis.
Viscolin Inhibits In Vitro Smooth Muscle Cell Proliferation and Migration and Neointimal Hyperplasia In Vivo.
Transplantation of IL-10-transfected endothelial progenitor cells improves retinal vascular repair via suppressing inflammation in diabetic rats.
Topological control of nitric oxide secretion by tantalum oxide nanodot arrays.
Inhibition of thioredoxin 1 leads to apoptosis in drug-resistant multiple myeloma.
Effects of intrauterine growth retardation and postnatal high-fat diet on hepatic inflammatory response in pigs.
Ganoderma lucidum Polysaccharides Reduce Lipopolysaccharide-Induced Interleukin-1 β Expression in Cultured Smooth Muscle Cells and in Thoracic Aortas in Mice.
Increased serum HMGB1 levels in patients with Henoch-Schönlein purpura.
Viscolin reduces VCAM-1 expression in TNF-α-treated endothelial cells via the JNK/NF-κB and ROS pathway.
Association of a functional variant downstream of TNFAIP3 with systemic lupus erythematosus.
Functional module analysis reveals differential osteogenic and stemness potentials in human mesenchymal stem cells from bone marrow and Wharton's jelly of umbilical cord.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
Monoacylglycerol lipase regulates cannabinoid receptor 2-dependent macrophage activation and cancer progression.
Xiang W, Shi R, Kang X, Zhang X, Chen P, Zhang L, Hou A, Wang R, Zhao Y, Zhao K, Liu Y, Ma Y, Luo H, Shang S, Zhang J, He F, Yu S, Gan L, Shi C, Li Y, Yang W, Liang H, Miao H
Nature communications 2018 Jul 3;9(1):2574
Nature communications 2018 Jul 3;9(1):2574
Acetylation state of RelA modulated by epigenetic drugs prolongs survival and induces a neuroprotective effect on ALS murine model.
Schiaffino L, Bonafede R, Scambi I, Parrella E, Pizzi M, Mariotti R
Scientific reports 2018 Aug 27;8(1):12875
Scientific reports 2018 Aug 27;8(1):12875
2,3,5,4'-Tetrahydroxystilbene-2-O-β-glucoside Isolated from Polygoni Multiflori Ameliorates the Development of Periodontitis.
Chin YT, Hsieh MT, Lin CY, Kuo PJ, Yang YC, Shih YJ, Lai HY, Cheng GY, Tang HY, Lee CC, Lee SY, Wang CC, Lin HY, Fu E, Whang-Peng J, Liu LF
Mediators of inflammation 2016;2016:6953459
Mediators of inflammation 2016;2016:6953459
Viscolin Inhibits In Vitro Smooth Muscle Cell Proliferation and Migration and Neointimal Hyperplasia In Vivo.
Chen CC, Liang CJ, Leu YL, Chen YL, Wang SH
PloS one 2016;11(12):e0168092
PloS one 2016;11(12):e0168092
Transplantation of IL-10-transfected endothelial progenitor cells improves retinal vascular repair via suppressing inflammation in diabetic rats.
Wang Y, Fan L, Meng X, Jiang F, Chen Q, Zhang Z, Yan H
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 2016 Oct;254(10):1957-1965
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 2016 Oct;254(10):1957-1965
Topological control of nitric oxide secretion by tantalum oxide nanodot arrays.
Dhawan U, Lee CH, Huang CC, Chu YH, Huang GS, Lin YR, Chen WL
Journal of nanobiotechnology 2015 Nov 9;13:79
Journal of nanobiotechnology 2015 Nov 9;13:79
Inhibition of thioredoxin 1 leads to apoptosis in drug-resistant multiple myeloma.
Raninga PV, Di Trapani G, Vuckovic S, Bhatia M, Tonissen KF
Oncotarget 2015 Jun 20;6(17):15410-24
Oncotarget 2015 Jun 20;6(17):15410-24
Effects of intrauterine growth retardation and postnatal high-fat diet on hepatic inflammatory response in pigs.
Liu J, He J, Yang Y, Yu J, Mao X, Yu B, Chen D
Archives of animal nutrition 2014;68(2):111-25
Archives of animal nutrition 2014;68(2):111-25
Ganoderma lucidum Polysaccharides Reduce Lipopolysaccharide-Induced Interleukin-1 β Expression in Cultured Smooth Muscle Cells and in Thoracic Aortas in Mice.
Liang CJ, Lee CW, Sung HC, Chen YH, Chiang YC, Hsu HY, Tseng YC, Li CY, Wang SH, Chen YL
Evidence-based complementary and alternative medicine : eCAM 2014;2014:305149
Evidence-based complementary and alternative medicine : eCAM 2014;2014:305149
Increased serum HMGB1 levels in patients with Henoch-Schönlein purpura.
Chen T, Guo ZP, Wang WJ, Qin S, Cao N, Li MM
Experimental dermatology 2014 Jun;23(6):419-23
Experimental dermatology 2014 Jun;23(6):419-23
Viscolin reduces VCAM-1 expression in TNF-α-treated endothelial cells via the JNK/NF-κB and ROS pathway.
Liang CJ, Wang SH, Chen YH, Chang SS, Hwang TL, Leu YL, Tseng YC, Li CY, Chen YL
Free radical biology & medicine 2011 Oct 1;51(7):1337-46
Free radical biology & medicine 2011 Oct 1;51(7):1337-46
Association of a functional variant downstream of TNFAIP3 with systemic lupus erythematosus.
Adrianto I, Wen F, Templeton A, Wiley G, King JB, Lessard CJ, Bates JS, Hu Y, Kelly JA, Kaufman KM, Guthridge JM, Alarcón-Riquelme ME, BIOLUPUS and GENLES Networks., Anaya JM, Bae SC, Bang SY, Boackle SA, Brown EE, Petri MA, Gallant C, Ramsey-Goldman R, Reveille JD, Vila LM, Criswell LA, Edberg JC, Freedman BI, Gregersen PK, Gilkeson GS, Jacob CO, James JA, Kamen DL, Kimberly RP, Martin J, Merrill JT, Niewold TB, Park SY, Pons-Estel BA, Scofield RH, Stevens AM, Tsao BP, Vyse TJ, Langefeld CD, Harley JB, Moser KL, Webb CF, Humphrey MB, Montgomery CG, Gaffney PM
Nature genetics 2011 Mar;43(3):253-8
Nature genetics 2011 Mar;43(3):253-8
Functional module analysis reveals differential osteogenic and stemness potentials in human mesenchymal stem cells from bone marrow and Wharton's jelly of umbilical cord.
Hsieh JY, Fu YS, Chang SJ, Tsuang YH, Wang HW
Stem cells and development 2010 Dec;19(12):1895-910
Stem cells and development 2010 Dec;19(12):1895-910
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Supportive validation
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- Sample(30 ?g whole cell lysate)A:MOLT4 (GTX27912)7.5% SDS PAGEGTX107678 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- NFkB p65 antibody detects NFkB p65 protein by western blot analysis.A. 30 ?g GL261 whole cell lysate/extract B. 30 ?g C8D30 whole cell lysate/extract C. 30 ?g NIH-3T3 whole cell lysate/extract 10% SDS-PAGENFkB p65 antibody (GTX107678) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- NFkB p65 antibody detects NFkB p65 protein by western blot analysis. Non-transfected (-) and NFkB p65 -transfected (+, including GFP-tag) 293T whole cell extracts (15 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted by 1:5000.
- Validation comment
- WB
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- NFkB p65 antibody detects NFkB p65 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g A431 whole cell lysate/extract 7.5% SDS-PAGENFkB p65 antibody (GTX107678) dilution: 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- Main image
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- NFkB p65 antibody detects NFkB p65 protein by western blot analysis.A. 30 ?g K562 whole cell lysate/extract7.5% SDS-PAGENFkB p65 antibody (GTX107678) dilution: 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NFkB p65 antibody detects NFkB p65 protein by western blot analysis. Non-transfected (-) and NFkB p65 -transfected (+, including GFP-tag) 293T whole cell extracts (15 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membranes were blotted with NFkB p65 antibody (GTX107678) diluted at 1:1000 and competitor's antibody (#4764) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.*The competitor is not affiliated with GeneTex and does not endorse this product.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NFkB p65 antibody (GTX107678) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
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- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using NFkB p65(GTX107678) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
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- NFkB p65 antibody detects NFkB p65 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NFkB p65 protein stained by NFkB p65 antibody (GTX107678) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining.
Supportive validation
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- NFkB p65 antibody immunoprecipitates NFkB p65 protein in IP experiments. IP Sample: 1000 ?g HeLa whole cell lysate/extract A. 40 £gg HeLa whole cell lysate/extract B. Control with 2.5 £gg of preimmune rabbit IgG C. Immunoprecipitation of NFkB p65 protein by 2.5 £gg of NFkB p65 antibody (GTX107678) 10% SDS-PAGE The immunoprecipitated NFkB p65 protein was detected by NFkB p65 antibody (GTX107678) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
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- Immunohistochemical analysis of paraffin-embedded SAS xenograft, using NFkB p65 (GTX107678) antibody at 1:500 dilution.