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Western blot
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C413576 - Provider product page
- Provider
- LSBio
- Product name
- RELA / NFKB p65 Antibody LS-C413576
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Storage
- Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of NF-kappaB p65 expression in HeLa (A); mouse spleen (B); rat heart (C) whole cell lysates.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitation of NF-kappaB p65 from 0.5mg HeLa whole cell extract lysate; using Anti-NF-kappaB p65 Antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation of NF-kappaB p65 from 0.5mg HeLa whole cell extract lysate; using Anti-NF-kappaB p65 Antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation of NF-kappaB p65 from 0.5mg HeLa whole cell extract lysate; using Anti-NF-kappaB p65 Antibody.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemical analysis of NF-kappaB p65 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
