LS-C413576

antibody from LSBio

Targeting: RELA NFKB3, p65

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C413576

Provider

LSBio

Product name

RELA / NFKB p65 Antibody LS-C413576

Antibody type

Monoclonal

Description

Purified

Reactivity

Human, Mouse, Rat

Host

Mouse

Storage

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitation of NF-kappaB p65 from 0.5mg HeLa whole cell extract lysate; using Anti-NF-kappaB p65 Antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation of NF-kappaB p65 from 0.5mg HeLa whole cell extract lysate; using Anti-NF-kappaB p65 Antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation of NF-kappaB p65 from 0.5mg HeLa whole cell extract lysate; using Anti-NF-kappaB p65 Antibody.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemical analysis of NF-kappaB p65 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.