Antibody data
- Antibody Data
- Antigen structure
- References [98]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- A00284-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-NF-kB p65/RELA Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for NF-kB p65 detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Baicalin-2-ethoxyethyl ester alleviates gentamicin-induced acute kidney injury via NF-κB signaling pathway.
Gut microbiota contribution to selenium deficiency-induced gut-liver inflammation.
Three Artemisia pollens trigger the onset of allergic rhinitis via TLR4/MyD88 signaling pathway.
Active fractions from Jingfang Baidu Powder alleviate Klebsiella-induced Pneumonia by inhibiting TLR4/Myd88-ERK signaling pathway.
Zafirlukast protects against hepatic ischemia-reperfusion injury in rats via modulating Bcl-2/Bax and NF-κB/SMAD-4 pathways.
Hippophae rhamnoides reverses decreased CYP2D6 expression in rats with BCG-induced liver injury.
Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration.
Sirt4 deficiency promotes the development of atherosclerosis by activating the NF-κB/IκB/CXCL2/3 pathway.
Acutely increased aquaporin-4 exhibits more potent protective effects in the cortex against single and repeated isoflurane-induced neurotoxicity in the developing rat brain.
Istradefylline induces A2A/P2X7 crosstalk expression inducing pro-inflammatory signal, and reduces AKT/mTOR signaling in melanoma-bearing mice.
Hsp27, Hsp60, Hsp70, or Hsp90 depletion enhances the antitumor effects of resveratrol via oxidative and ER stress response in human glioblastoma cells.
Mechanism investigation of Duhuo Jisheng pill against rheumatoid arthritis based on a strategy for the integration of network pharmacology, molecular docking and in vivo experimental verification.
UHPLC-MS-Based Metabolomics Reveal the Potential Mechanism of Armillaria mellea Acid Polysaccharide in and Its Effects on Cyclophosphamide-Induced Immunosuppressed Mice.
Bisphenol A exposure exacerbates tracheal inflammatory injury in selenium-deficient chickens by regulating the miR-155/TRAF3/ROS pathway.
Active ingredients screening and pharmacological mechanism research of curcumae rhizoma-sparganii rhizoma herb pair ameliorates liver fibrosis based on network pharmacology.
Leonurine Preconditioning Attenuates Ischemic Acute Kidney Injury in Rats by Promoting Nrf2 Nuclear Translocation and Suppressing TLR4/NF-κB Pathway.
Human Neural Stem Cell Secretome Inhibits Lipopolysaccharide-Induced Neuroinflammation Through Modulating Microglia Polarization by Activating Peroxisome Proliferator-Activated Receptor Gamma.
Intravenous immunoglobulin is effective in alleviating hepatic ischemia-reperfusion injury: a rat model study.
Triptolide attenuates LPS-induced activation of RAW 264.7 macrophages by inducing M1-to-M2 repolarization via the mTOR/STAT3 signaling.
An Acidic Polysaccharide with Anti-Inflammatory Effects from Blackened Jujube: Conformation and Rheological Properties.
The antimicrobial peptide YD attenuates inflammation via miR-155 targeting CASP12 during liver fibrosis.
Gastroprotective Activity of the Total Flavones from Abelmoschus manihot (L.) Medic Flowers.
Liuweidihuang Pill Alleviates Inflammation of the Testis via AMPK/SIRT1/NF-κB Pathway in Aging Rats.
MicroRNA134 of Ventral Hippocampus Is Involved in Cocaine Extinction-Induced Anxiety-like and Depression-like Behaviors in Mice.
PC-3-Derived Exosomes Inhibit Osteoclast Differentiation by Downregulating miR-214 and Blocking NF-κB Signaling Pathway.
Partial liquid ventilation-induced mild hypothermia improves the lung function and alleviates the inflammatory response during acute respiratory distress syndrome in canines.
Bone marrow stromal cells improved functional recovery in spinal cord injury rats partly via the Toll-like receptor-4/nuclear factor-κB signaling pathway.
Comparison of adipose‑ and bone marrow‑derived stem cells in protecting against ox‑LDL‑induced inflammation in M1‑macrophage‑derived foam cells.
Diosgenin Attenuates Lipopolysaccharide-Induced Parkinson's Disease by Inhibiting the TLR/NF-κB Pathway.
Adolescent cocaine exposure induces prolonged synaptic modifications in medial prefrontal cortex of adult rats.
TNF-α-induced NF-κB activation promotes myofibroblast differentiation of LR-MSCs and exacerbates bleomycin-induced pulmonary fibrosis.
Sophocarpine attenuates wear particle-induced implant loosening by inhibiting osteoclastogenesis and bone resorption via suppression of the NF-κB signalling pathway in a rat model.
Angiotensin-(1-7)-mediated Mas1 receptor/NF-κB-p65 signaling is involved in a cigarette smoke-induced chronic obstructive pulmonary disease mouse model.
Tenacissoside H exerts an anti-inflammatory effect by regulating the nf-κb and p38 pathways in zebrafish.
Celastrol Attenuates Multiple Sclerosis and Optic Neuritis in an Experimental Autoimmune Encephalomyelitis Model.
Glaucocalyxin B Alleviates Lipopolysaccharide-Induced Parkinson's Disease by Inhibiting TLR/NF-κB and Activating Nrf2/HO-1 Pathway.
Andrographolide Inhibits Inflammatory Cytokines Secretion in LPS-Stimulated RAW264.7 Cells through Suppression of NF-κB/MAPK Signaling Pathway.
Pharmacological Effect of Caulophyllum robustum on Collagen-Induced Arthritis and Regulation of Nitric Oxide, NF-κB, and Proinflammatory Cytokines In Vivo and In Vitro.
The Antigastric Cancer Activity of San Leng Powder Extract Induces Apoptosis in Balb/C Bearing-SGC-7901 Mice.
Cinnamon extracts exert intrapancreatic cytoprotection against streptozotocin in vivo.
Naringin attenuates diabetic retinopathy by inhibiting inflammation, oxidative stress and NF-κB activation in vivo and in vitro.
Nephroprotective Effects of Saponins from Leaves of Panax quinquefolius against Cisplatin-Induced Acute Kidney Injury.
Prunella vulgaris L., an Edible and Medicinal Plant, Attenuates Scopolamine-Induced Memory Impairment in Rats.
Effect of curcumin on expressions of NF-κBp65, TNF-α and IL-8 in placental tissue of premature birth of infected mice.
Silencing of carboxypeptidase E inhibits cell proliferation, tumorigenicity, and metastasis of osteosarcoma cells.
Overexpression of Livin promotes migration and invasion of colorectal cancer cells by induction of epithelial-mesenchymal transition via NF-κB activation.
Characterization of acetaminophen-induced cytotoxicity in target tissues.
Puerarin Improves Diabetic Aorta Injury by Inhibiting NADPH Oxidase-Derived Oxidative Stress in STZ-Induced Diabetic Rats.
Glycyrrhizic Acid Attenuates Sepsis-Induced Acute Kidney Injury by Inhibiting NF-κB Signaling Pathway.
BMSCs Interactions with Adventitial Fibroblasts Display Smooth Muscle Cell Lineage Potential in Differentiation and Migration That Contributes to Neointimal Formation.
Hydrogen Sulfide Mitigates Kidney Injury in High Fat Diet-Induced Obese Mice.
Differences in Proinflammatory Property of Six Subtypes of Peroxiredoxins and Anti-Inflammatory Effect of Ligustilide in Macrophages.
Cerebralcare Granule(®), a Chinese Herb Compound Preparation, Attenuates D-Galactose Induced Memory Impairment in Mice.
Knockdown of placental growth factor (PLGF) mitigates hyperoxia-induced acute lung injury in neonatal rats: Suppressive effects on NFκB signaling pathway.
Placental Growth Factor Triggers Epithelial-to-Mesenchymal Transition-like Changes in Rat Type II Alveolar Epithelial Cells: Activation of Nuclear Factor κB Signalling Pathway.
Ursolic acid protects against ulcerative colitis via anti-inflammatory and antioxidant effects in mice.
Anti-inflammatory and antioxidant effects of curcumin on acute lung injury in a rodent model of intestinal ischemia reperfusion by inhibiting the pathway of NF-Kb.
Effects of PDTC on NF-κB expression and apoptosis in rats with severe acute pancreatitis-associated lung injury.
Curcumin ameliorates asthmatic airway inflammation by activating nuclear factor-E2-related factor 2/haem oxygenase (HO)-1 signalling pathway.
Pyrrolidine Dithiocarbamate Inhibits Nuclear Factor κB and Toll-Like Receptor 4 Expression in Rats with Acute Necrotizing Pancreatitis.
Probucol prevents atrial remodeling by inhibiting oxidative stress and TNF-α/NF-κB/TGF-β signal transduction pathway in alloxan-induced diabetic rabbits.
Down-regulation of HMGB1 expression by shRNA constructs inhibits the bioactivity of urothelial carcinoma cell lines via the NF-κB pathway.
Effects of hydroxysafflor yellow A on proliferation and collagen synthesis of rat vascular adventitial fibroblasts induced by angiotensin II.
Ox-LDL influences peripheral Th17/Treg balance by modulating Treg apoptosis and Th17 proliferation in atherosclerotic cerebral infarction.
The anti-inflammation effect of baicalin on experimental colitis through inhibiting TLR4/NF-κB pathway activation.
Kallistatin, a novel anti-angiogenesis agent, inhibits angiogenesis via inhibition of the NF-κB signaling pathway.
Anti-fatigue and vasoprotective effects of quercetin-3-O-gentiobiose on oxidative stress and vascular endothelial dysfunction induced by endurance swimming in rats.
Treatment of low molecular weight heparin inhibits systemic inflammation and prevents endotoxin-induced acute lung injury in rats.
Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.
Antiviral effects of modified dingchuan decoction against respiratory syncytial virus infection in vitro and in an immunosuppressive mouse model.
Paeonol inhibits hepatic fibrogenesis via disrupting nuclear factor-κB pathway in activated stellate cells: in vivo and in vitro studies.
Dietary quercetin ameliorates nonalcoholic steatohepatitis induced by a high-fat diet in gerbils.
Protective effects of glutamine in a rat model of endotoxemia.
Anti-inflammatory and immunomodulatory effects of iridoid glycosides from Paederia scandens (LOUR.) MERRILL (Rubiaceae) on uric acid nephropathy rats.
Calcitonin gene-related peptide released from endothelial progenitor cells inhibits the proliferation of rat vascular smooth muscle cells induced by angiotensin II.
Extract of the dried heartwood of Caesalpinia sappan L. attenuates collagen-induced arthritis.
The effect of endothelial progenitor cells on angiotensin II-induced proliferation of cultured rat vascular smooth muscle cells.
Protective effect and mechanism of sodium tanshinone II A sulfonate on microcirculatory disturbance of small intestine in rats with sepsis.
Growth hormone releasing peptide-2, a ghrelin agonist, attenuates lipopolysaccharide-induced acute lung injury in rats.
Role of intrapulmonary expression of inducible nitric oxide synthase gene and nuclear factor kappaB activation in severe pancreatitis-associated lung injury.
Oligomannurarate sulfate blocks tumor growth by inhibiting NF-kappaB activation.
Tanshinone II A attenuates inflammatory responses of rats with myocardial infarction by reducing MCP-1 expression.
Histone deacetylase inhibitor, butyrate, attenuates lipopolysaccharide-induced acute lung injury in mice.
Apoptotic mechanism of MCF-7 breast cells in vivo and in vitro induced by photodynamic therapy with C-phycocyanin.
Norisoboldine inhibits the production of pro-inflammatory cytokines in lipopolysaccharide-stimulated RAW 264.7 cells by down-regulating the activation of MAPKs but not NF-κB.
Effect of Dachengqi decoction on NF-kappaB p65 expression in lung of rats with partial intestinal obstruction and the underlying mechanism.
Lipoxin A4 analogue protects brain and reduces inflammation in a rat model of focal cerebral ischemia reperfusion.
Neuroprotective effects of Tanshinone IIA on permanent focal cerebral ischemia in mice.
Effects of triptolide from Radix Tripterygium wilfordii (Leigongteng) on cartilage cytokines and transcription factor NF-kappaB: a study on induced arthritis in rats.
Essential role for nuclear factor kappaB in ischemic preconditioning for cold ischemia-reperfusion injury of intestinal transplantation.
Heparanase expression in gallbladder carcinoma and its correlation to prognosis.
Effects of traditional chinese medicine on endotoxin and its receptors in rats with non-alcoholic steatohepatitis.
Heme oxygenase-1 induction by hemin protects liver cells from ischemia/reperfusion injury in cirrhotic rats.
Angiotensin-converting enzyme inhibitor captopril prevents oleic acid-induced severe acute lung injury in rats.
Triptolide inhibits NF-kappaB activation and reduces injury of donor lung induced by ischemia/reperfusion.
Prolactin modulates the functions of murine spleen CD11c-positive dendritic cells.
Progesterone is involved in the maturation of murine spleen CD11c-positive dendritic cells.
Effects of 17beta-estradiol on the maturation, nuclear factor kappa B p65 and functions of murine spleen CD11c-positive dendritic cells.
Chen W, Zhang K, Cui H, Li J, Yao D, Wang S, Tian X, Kou W, Huang J, Wang H, Wang J
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024 Mar;172:116276
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024 Mar;172:116276
Gut microbiota contribution to selenium deficiency-induced gut-liver inflammation.
Wang G, Jiang Z, Song Y, Xing Y, He S, Boomi P
BioFactors (Oxford, England) 2024 Mar-Apr;50(2):311-325
BioFactors (Oxford, England) 2024 Mar-Apr;50(2):311-325
Three Artemisia pollens trigger the onset of allergic rhinitis via TLR4/MyD88 signaling pathway.
Zhang J, Gao L, Yu D, Song Y, Zhao Y, Feng Y
Molecular biology reports 2024 Feb 23;51(1):319
Molecular biology reports 2024 Feb 23;51(1):319
Active fractions from Jingfang Baidu Powder alleviate Klebsiella-induced Pneumonia by inhibiting TLR4/Myd88-ERK signaling pathway.
Sun C, Xu Y, Xu G, Ji X, Jiang P, He Y
Journal of ethnopharmacology 2024 Aug 10;330:118067
Journal of ethnopharmacology 2024 Aug 10;330:118067
Zafirlukast protects against hepatic ischemia-reperfusion injury in rats via modulating Bcl-2/Bax and NF-κB/SMAD-4 pathways.
Mahmoud HM, Elsayed Abouzed DE, Abo-Youssef AM, Hemeida RAM
International immunopharmacology 2023 Sep;122:110498
International immunopharmacology 2023 Sep;122:110498
Hippophae rhamnoides reverses decreased CYP2D6 expression in rats with BCG-induced liver injury.
Zou H, Hao P, Cao Y, Li L, Ding R, Bai X, Xue Y
Scientific reports 2023 Oct 13;13(1):17425
Scientific reports 2023 Oct 13;13(1):17425
Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration.
Tan J, Zhuo Z, Wang X, Zhang Y, Qian Y, Lin F
International journal of molecular medicine 2023 Nov;52(5)
International journal of molecular medicine 2023 Nov;52(5)
Sirt4 deficiency promotes the development of atherosclerosis by activating the NF-κB/IκB/CXCL2/3 pathway.
Chang S, Zhang G, Li L, Li H, Jin X, Wang Y, Li B
Atherosclerosis 2023 May;373:29-37
Atherosclerosis 2023 May;373:29-37
Acutely increased aquaporin-4 exhibits more potent protective effects in the cortex against single and repeated isoflurane-induced neurotoxicity in the developing rat brain.
Yılmaz H, Şengelen A, Demirgan S, Paşaoğlu HE, Çağatay M, Erman İE, Bay M, Güneyli HC, Önay-Uçar E
Toxicology mechanisms and methods 2023 May;33(4):279-292
Toxicology mechanisms and methods 2023 May;33(4):279-292
Istradefylline induces A2A/P2X7 crosstalk expression inducing pro-inflammatory signal, and reduces AKT/mTOR signaling in melanoma-bearing mice.
Gutknecht da Silva JL, Passos DF, Cabral FL, Miron VV, Schetinger MRC, Cardoso AA, Dal Piva CH, Gomes CO, Ebone RS, Leal DBR
Medical oncology (Northwood, London, England) 2023 May 15;40(6):178
Medical oncology (Northwood, London, England) 2023 May 15;40(6):178
Hsp27, Hsp60, Hsp70, or Hsp90 depletion enhances the antitumor effects of resveratrol via oxidative and ER stress response in human glioblastoma cells.
Önay Uçar E, Şengelen A, Mertoğlu Kamalı E
Biochemical pharmacology 2023 Feb;208:115409
Biochemical pharmacology 2023 Feb;208:115409
Mechanism investigation of Duhuo Jisheng pill against rheumatoid arthritis based on a strategy for the integration of network pharmacology, molecular docking and in vivo experimental verification.
Xin P, Xu X, Zhang H, Hu Y, Deng C, Sun S, Liu S, Zhou X, Ma H, Li X
Pharmaceutical biology 2023 Dec;61(1):1431-1445
Pharmaceutical biology 2023 Dec;61(1):1431-1445
UHPLC-MS-Based Metabolomics Reveal the Potential Mechanism of Armillaria mellea Acid Polysaccharide in and Its Effects on Cyclophosphamide-Induced Immunosuppressed Mice.
Li Y, Li Q, Niu H, Li H, Jiao L, Wu W
Molecules (Basel, Switzerland) 2023 Dec 5;28(24)
Molecules (Basel, Switzerland) 2023 Dec 5;28(24)
Bisphenol A exposure exacerbates tracheal inflammatory injury in selenium-deficient chickens by regulating the miR-155/TRAF3/ROS pathway.
Peng L, Chen S, Lin H, Wan C, Li X, Xu S, Li S
International journal of biological macromolecules 2023 Dec 31;253(Pt 8):127501
International journal of biological macromolecules 2023 Dec 31;253(Pt 8):127501
Active ingredients screening and pharmacological mechanism research of curcumae rhizoma-sparganii rhizoma herb pair ameliorates liver fibrosis based on network pharmacology.
Hao M, Yao Z, Zhao M, Chen Z, Wang P, Sang X, Yang Q, Wang K, Han X, Cao G
Journal of ethnopharmacology 2023 Apr 6;305:116111
Journal of ethnopharmacology 2023 Apr 6;305:116111
Leonurine Preconditioning Attenuates Ischemic Acute Kidney Injury in Rats by Promoting Nrf2 Nuclear Translocation and Suppressing TLR4/NF-κB Pathway.
Han L, Chen A, Liu L, Wang F
Chemical & pharmaceutical bulletin 2022;70(1):66-73
Chemical & pharmaceutical bulletin 2022;70(1):66-73
Human Neural Stem Cell Secretome Inhibits Lipopolysaccharide-Induced Neuroinflammation Through Modulating Microglia Polarization by Activating Peroxisome Proliferator-Activated Receptor Gamma.
Zhou J, Ni W, Ling Y, Lv X, Niu D, Zeng Y, Qiu Y, Si Y, Wang Z, Hu J
Stem cells and development 2022 Jul;31(13-14):369-382
Stem cells and development 2022 Jul;31(13-14):369-382
Intravenous immunoglobulin is effective in alleviating hepatic ischemia-reperfusion injury: a rat model study.
Chen Z, Lin R, Zhuo H, Xu F, Liu X
Molecular biology reports 2022 Jan;49(1):341-349
Molecular biology reports 2022 Jan;49(1):341-349
Triptolide attenuates LPS-induced activation of RAW 264.7 macrophages by inducing M1-to-M2 repolarization via the mTOR/STAT3 signaling.
Zhu H, Tong S, Yan C, Zhou A, Wang M, Li C
Immunopharmacology and immunotoxicology 2022 Dec;44(6):894-901
Immunopharmacology and immunotoxicology 2022 Dec;44(6):894-901
An Acidic Polysaccharide with Anti-Inflammatory Effects from Blackened Jujube: Conformation and Rheological Properties.
Liu C, Wang F, Zhang R
Foods (Basel, Switzerland) 2022 Aug 17;11(16)
Foods (Basel, Switzerland) 2022 Aug 17;11(16)
The antimicrobial peptide YD attenuates inflammation via miR-155 targeting CASP12 during liver fibrosis.
Yan Z, Wang D, An C, Xu H, Zhao Q, Shi Y, Song N, Deng B, Guo X, Rao J, Cheng L, Zhang B, Mou L, Yang W, Jiang X, Xie J
Acta pharmaceutica Sinica. B 2021 Jan;11(1):100-111
Acta pharmaceutica Sinica. B 2021 Jan;11(1):100-111
Gastroprotective Activity of the Total Flavones from Abelmoschus manihot (L.) Medic Flowers.
Zhang J, Fu ZL, Chu ZX, Song BW
Evidence-based complementary and alternative medicine : eCAM 2020;2020:6584945
Evidence-based complementary and alternative medicine : eCAM 2020;2020:6584945
Liuweidihuang Pill Alleviates Inflammation of the Testis via AMPK/SIRT1/NF-κB Pathway in Aging Rats.
Wang Y, Yang Z, Yang L, Zou Q, Zhao S, Hu N, Chen D, Cui R, Ma H
Evidence-based complementary and alternative medicine : eCAM 2020;2020:2792738
Evidence-based complementary and alternative medicine : eCAM 2020;2020:2792738
MicroRNA134 of Ventral Hippocampus Is Involved in Cocaine Extinction-Induced Anxiety-like and Depression-like Behaviors in Mice.
Li Y, Lu X, Nie J, Hu P, Ge F, Yuan TF, Guan X
Molecular therapy. Nucleic acids 2020 Mar 6;19:937-950
Molecular therapy. Nucleic acids 2020 Mar 6;19:937-950
PC-3-Derived Exosomes Inhibit Osteoclast Differentiation by Downregulating miR-214 and Blocking NF-κB Signaling Pathway.
Duan Y, Tan Z, Yang M, Li J, Liu C, Wang C, Zhang F, Jin Y, Wang Y, Zhu L
BioMed research international 2019;2019:8650846
BioMed research international 2019;2019:8650846
Partial liquid ventilation-induced mild hypothermia improves the lung function and alleviates the inflammatory response during acute respiratory distress syndrome in canines.
Wei F, Wen S, Wu H, Ma L, Huang Y, Yang L
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Oct;118:109344
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Oct;118:109344
Bone marrow stromal cells improved functional recovery in spinal cord injury rats partly via the Toll-like receptor-4/nuclear factor-κB signaling pathway.
Bai S, Zhou H, Wu L
Experimental and therapeutic medicine 2019 Jan;17(1):444-448
Experimental and therapeutic medicine 2019 Jan;17(1):444-448
Comparison of adipose‑ and bone marrow‑derived stem cells in protecting against ox‑LDL‑induced inflammation in M1‑macrophage‑derived foam cells.
Li JZ, Cao TH, Han JC, Qu H, Jiang SQ, Xie BD, Yan XL, Wu H, Liu XL, Zhang F, Leng XP, Kang K, Jiang SL
Molecular medicine reports 2019 Apr;19(4):2660-2670
Molecular medicine reports 2019 Apr;19(4):2660-2670
Diosgenin Attenuates Lipopolysaccharide-Induced Parkinson's Disease by Inhibiting the TLR/NF-κB Pathway.
Li B, Xu P, Wu S, Jiang Z, Huang Z, Li Q, Chen D
Journal of Alzheimer's disease : JAD 2018;64(3):943-955
Journal of Alzheimer's disease : JAD 2018;64(3):943-955
Adolescent cocaine exposure induces prolonged synaptic modifications in medial prefrontal cortex of adult rats.
Zhu W, Ge X, Gao P, Li M, Guan Y, Guan X
Brain structure & function 2018 May;223(4):1829-1838
Brain structure & function 2018 May;223(4):1829-1838
TNF-α-induced NF-κB activation promotes myofibroblast differentiation of LR-MSCs and exacerbates bleomycin-induced pulmonary fibrosis.
Hou J, Ma T, Cao H, Chen Y, Wang C, Chen X, Xiang Z, Han X
Journal of cellular physiology 2018 Mar;233(3):2409-2419
Journal of cellular physiology 2018 Mar;233(3):2409-2419
Sophocarpine attenuates wear particle-induced implant loosening by inhibiting osteoclastogenesis and bone resorption via suppression of the NF-κB signalling pathway in a rat model.
Zhou CH, Shi ZL, Meng JH, Hu B, Zhao CC, Yang YT, Yu W, Chen ZX, Heng BC, Parkman VA, Jiang S, Zhu HX, Wu HB, Shen WL, Yan SG
British journal of pharmacology 2018 Mar;175(6):859-876
British journal of pharmacology 2018 Mar;175(6):859-876
Angiotensin-(1-7)-mediated Mas1 receptor/NF-κB-p65 signaling is involved in a cigarette smoke-induced chronic obstructive pulmonary disease mouse model.
Zhang Y, Li Y, Shi C, Fu X, Zhao L, Song Y
Environmental toxicology 2018 Jan;33(1):5-15
Environmental toxicology 2018 Jan;33(1):5-15
Tenacissoside H exerts an anti-inflammatory effect by regulating the nf-κb and p38 pathways in zebrafish.
Li JJ, Zhang Y, Han LW, Tian QP, He QX, Wang XM, Sun C, Han J, Liu KC
Fish & shellfish immunology 2018 Dec;83:205-212
Fish & shellfish immunology 2018 Dec;83:205-212
Celastrol Attenuates Multiple Sclerosis and Optic Neuritis in an Experimental Autoimmune Encephalomyelitis Model.
Yang H, Liu C, Jiang J, Wang Y, Zhang X
Frontiers in pharmacology 2017;8:44
Frontiers in pharmacology 2017;8:44
Glaucocalyxin B Alleviates Lipopolysaccharide-Induced Parkinson's Disease by Inhibiting TLR/NF-κB and Activating Nrf2/HO-1 Pathway.
Xu W, Zheng D, Liu Y, Li J, Yang L, Shang X
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;44(6):2091-2104
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;44(6):2091-2104
Andrographolide Inhibits Inflammatory Cytokines Secretion in LPS-Stimulated RAW264.7 Cells through Suppression of NF-κB/MAPK Signaling Pathway.
Li Y, He S, Tang J, Ding N, Chu X, Cheng L, Ding X, Liang T, Feng S, Rahman SU, Wang X, Wu J
Evidence-based complementary and alternative medicine : eCAM 2017;2017:8248142
Evidence-based complementary and alternative medicine : eCAM 2017;2017:8248142
Pharmacological Effect of Caulophyllum robustum on Collagen-Induced Arthritis and Regulation of Nitric Oxide, NF-κB, and Proinflammatory Cytokines In Vivo and In Vitro.
Wang QH, Lv SW, Guo YY, Duan JX, Dong SY, Wang QS, Yu FM, Su H, Kuang HX
Evidence-based complementary and alternative medicine : eCAM 2017;2017:8134321
Evidence-based complementary and alternative medicine : eCAM 2017;2017:8134321
The Antigastric Cancer Activity of San Leng Powder Extract Induces Apoptosis in Balb/C Bearing-SGC-7901 Mice.
Yu C, Ji AJ, Lu JW, Liu SJ, Sun Y
Evidence-based complementary and alternative medicine : eCAM 2017;2017:1052125
Evidence-based complementary and alternative medicine : eCAM 2017;2017:1052125
Cinnamon extracts exert intrapancreatic cytoprotection against streptozotocin in vivo.
Liao Z, Wang J, Tan H, Wei L
Gene 2017 Sep 5;627:519-523
Gene 2017 Sep 5;627:519-523
Naringin attenuates diabetic retinopathy by inhibiting inflammation, oxidative stress and NF-κB activation in vivo and in vitro.
Liu L, Zuo Z, Lu S, Liu A, Liu X
Iranian journal of basic medical sciences 2017 Jul;20(7):813-821
Iranian journal of basic medical sciences 2017 Jul;20(7):813-821
Nephroprotective Effects of Saponins from Leaves of Panax quinquefolius against Cisplatin-Induced Acute Kidney Injury.
Ma ZN, Li YZ, Li W, Yan XT, Yang G, Zhang J, Zhao LC, Yang LM
International journal of molecular sciences 2017 Jul 13;18(7)
International journal of molecular sciences 2017 Jul 13;18(7)
Prunella vulgaris L., an Edible and Medicinal Plant, Attenuates Scopolamine-Induced Memory Impairment in Rats.
Qu Z, Zhang J, Yang H, Gao J, Chen H, Liu C, Gao W
Journal of agricultural and food chemistry 2017 Jan 18;65(2):291-300
Journal of agricultural and food chemistry 2017 Jan 18;65(2):291-300
Effect of curcumin on expressions of NF-κBp65, TNF-α and IL-8 in placental tissue of premature birth of infected mice.
Guo YZ, He P, Feng AM
Asian Pacific journal of tropical medicine 2017 Feb;10(2):175-178
Asian Pacific journal of tropical medicine 2017 Feb;10(2):175-178
Silencing of carboxypeptidase E inhibits cell proliferation, tumorigenicity, and metastasis of osteosarcoma cells.
Fan S, Li X, Li L, Wang L, Du Z, Yang Y, Zhao J, Li Y
OncoTargets and therapy 2016;9:2795-803
OncoTargets and therapy 2016;9:2795-803
Overexpression of Livin promotes migration and invasion of colorectal cancer cells by induction of epithelial-mesenchymal transition via NF-κB activation.
Ge Y, Cao X, Wang D, Sun W, Sun H, Han B, Cui J, Liu B
OncoTargets and therapy 2016;9:1011-21
OncoTargets and therapy 2016;9:1011-21
Characterization of acetaminophen-induced cytotoxicity in target tissues.
Guo C, Xie G, Su M, Wu X, Lu X, Wu K, Wei C
American journal of translational research 2016;8(10):4440-4445
American journal of translational research 2016;8(10):4440-4445
Puerarin Improves Diabetic Aorta Injury by Inhibiting NADPH Oxidase-Derived Oxidative Stress in STZ-Induced Diabetic Rats.
Li W, Zhao W, Wu Q, Lu Y, Shi J, Chen X
Journal of diabetes research 2016;2016:8541520
Journal of diabetes research 2016;2016:8541520
Glycyrrhizic Acid Attenuates Sepsis-Induced Acute Kidney Injury by Inhibiting NF-κB Signaling Pathway.
Zhao H, Zhao M, Wang Y, Li F, Zhang Z
Evidence-based complementary and alternative medicine : eCAM 2016;2016:8219287
Evidence-based complementary and alternative medicine : eCAM 2016;2016:8219287
BMSCs Interactions with Adventitial Fibroblasts Display Smooth Muscle Cell Lineage Potential in Differentiation and Migration That Contributes to Neointimal Formation.
Wendan Y, Changzhu J, Xuhong S, Hongjing C, Hong S, Dongxia Y, Fang X
Stem cells international 2016;2016:3196071
Stem cells international 2016;2016:3196071
Hydrogen Sulfide Mitigates Kidney Injury in High Fat Diet-Induced Obese Mice.
Wu D, Gao B, Li M, Yao L, Wang S, Chen M, Li H, Ma C, Ji A, Li Y
Oxidative medicine and cellular longevity 2016;2016:2715718
Oxidative medicine and cellular longevity 2016;2016:2715718
Differences in Proinflammatory Property of Six Subtypes of Peroxiredoxins and Anti-Inflammatory Effect of Ligustilide in Macrophages.
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- Experimental details
- Western blot analysis of NF-kB p65 using anti-NF-kB p65 antibody (A00284-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates,Lane 2: human A549 whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human MDA-MB-231 whole cell lysates,Lane 5: human PANC-1 whole cell lysates,Lane 6: human A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF-kB p65 antigen affinity purified polyclonal antibody (Catalog # A00284-1) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF-kB p65 at approximately 65KD. The expected band size for NF-kB p65 is at 65KD.
- Additional image