bs-3018R

antibody from Bioss

Targeting: EIF4EBP1 4E-BP1, PHAS-I

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

bs-3018R

Provider

Bioss

Proper citation

Bioss Cat#bs-3018R, RRID:AB_10860214

Product name

eIF4EBP1 (Ser65) Polyclonal Antibody

Antibody type

Polyclonal

Description

KLH conjugated synthetic phosphopeptide derived from mouse 4EBP1 around the phosphorylation site of Ser65

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100ul

Concentration

1ug/ul

Storage

Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

Western blot

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

Lane 1: mouse kidney lysates Lane 2: mouse embryo lysates probed with Anti phospho-eIF4EBP1/4EBP1(Ser64) Polyclonal Antibody, Unconjugated (bs-3018R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37°C. Predicted band 13kD. Observed band size: 13kD

Sample type

Mouse

Other comments

Kidney

Immunohistochemistry

Supportive validation

Submitted by

Bioss (provider)

Main image

Experimental details

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-eIF4EBP1(Ser64)Polyclonal Antibody, Unconjugated (bs-3018R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Sample type

Human

Other comments

Lung