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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
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- Product number
- GTX116315 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX116315, RRID:AB_10632047
- Product name
- 4E-BP1 antibody [N1C3-2]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T 15% SDS PAGE GTX116315 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- 4E-BP1 antibody detects 4E-BP1 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody (GTX116315) diluted at a dilution of 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3-2] (GTX116315) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3-2] (GTX116315) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- 4E-BP1 antibody [N1C3-2] detects 4E-BP1 protein at cytoplasm by immunofluorescent analysis.Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: 4E-BP1 protein stained by 4E-BP1 antibody [N1C3-2] (GTX116315) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
