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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-37557 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-4EBP1 (Thr46) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody detects endogenous level of 4E-BP1 only when phosphorylated at Threonine 46. A suggested positive control for Western blot is Hela cells; suggested positive control for IHC is human breast carcinoma.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from Hela cells using 4E-BP1 (pThr45) polyclonal antibody (Product # PA5-37557) compared to the same antibody preincubated with a blocking peptide (Lane 1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-4EBP1 (Thr46) in paraffin-embedded Human breast carcinoma tissue using Phospho-4EBP1 (Thr46) Polyclonal Antibody (Product # PA5-37557) (left) or the same antibody preincubated with blocking peptide (right).
