GTX55010

antibody from GeneTex

Targeting: EIF4EBP1 4E-BP1, PHAS-I

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX55010

Provider

GeneTex

Product name

4E-BP1 (phospho Thr69) antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Storage

Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of 4EBP1 (pT69) expression in HEK293T (A); NS-1 (B); H9C2 (C); rat liver (D) whole cell lysates.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of 4EBP1 (pT69) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.