GTX133184

antibody from GeneTex

Targeting: EIF4EBP1 4E-BP1, PHAS-I

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX133184

Provider

GeneTex

Product name

4E-BP1 (phospho Ser65) antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Untreated (¡V) and treated (+) 293T whole cell extracts (60 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Ser65) antibody (GTX133184) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and 4E-BP1 (phospho Ser65) knockout (KO) HeLa cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Ser65) antibody (GTX133184) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and 4E-BP1 (phospho Ser65) knockout (KO) HeLa cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Ser65) antibody (GTX133184) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.