Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 50-9107-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-4EBP1 (Thr36, Thr45) Monoclonal Antibody (V3NTY24), eFluor™ 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The V3NTY24 monoclonal antibody recognizes human and mouse eukaryotic translation initiation factor eIF4E-binding protein 1 (4E-BP1) when phosphorylated at threonine 37 and/or threonine 46. 4E-BP1 is a member of a family of translation repressor proteins that include 4E-BP2 and 4E-BP3. In its non-phosphorylated form, 4E-BP1 binds to the eIF4E translation initiation factor and represses cap-dependent translation. Phosphorylation of 4E-BP1 at multiple sites is necessary to disrupt this interaction and de-repress cap-dependent translation. Studies have identified several kinases that phosphorylate 4E-BP1. For instance, FRAP/mTOR phosphorylates Thr37 and Thr46, while ATM phosphorylates Ser111. Phosphorylation of 4E-BP1 at Thr37 and Thr46 is inhibited by the PI3 kinase inhibitors LY294002 and wortmannin.
- Antibody clone number
- V3NTY24
- Concentration
- 5 µL/Test
Submitted references IFNα Impairs Autophagic Degradation of mtDNA Promoting Autoreactivity of SLE Monocytes in a STING-Dependent Fashion.
Cell size and fat content of dietary-restricted Caenorhabditis elegans are regulated by ATX-2, an mTOR repressor.
Regulation of 4E-BP1 phosphorylation: a novel two-step mechanism.
4E-BP1, a repressor of mRNA translation, is phosphorylated and inactivated by the Akt(PKB) signaling pathway.
Phosphorylation of the translational repressor PHAS-I by the mammalian target of rapamycin.
Gkirtzimanaki K, Kabrani E, Nikoleri D, Polyzos A, Blanas A, Sidiropoulos P, Makrigiannakis A, Bertsias G, Boumpas DT, Verginis P
Cell reports 2018 Oct 23;25(4):921-933.e5
Cell reports 2018 Oct 23;25(4):921-933.e5
Cell size and fat content of dietary-restricted Caenorhabditis elegans are regulated by ATX-2, an mTOR repressor.
Bar DZ, Charar C, Dorfman J, Yadid T, Tafforeau L, Lafontaine DL, Gruenbaum Y
Proceedings of the National Academy of Sciences of the United States of America 2016 Aug 9;113(32):E4620-9
Proceedings of the National Academy of Sciences of the United States of America 2016 Aug 9;113(32):E4620-9
Regulation of 4E-BP1 phosphorylation: a novel two-step mechanism.
Gingras AC, Gygi SP, Raught B, Polakiewicz RD, Abraham RT, Hoekstra MF, Aebersold R, Sonenberg N
Genes & development 1999 Jun 1;13(11):1422-37
Genes & development 1999 Jun 1;13(11):1422-37
4E-BP1, a repressor of mRNA translation, is phosphorylated and inactivated by the Akt(PKB) signaling pathway.
Gingras AC, Kennedy SG, O'Leary MA, Sonenberg N, Hay N
Genes & development 1998 Feb 15;12(4):502-13
Genes & development 1998 Feb 15;12(4):502-13
Phosphorylation of the translational repressor PHAS-I by the mammalian target of rapamycin.
Brunn GJ, Hudson CC, Sekulić A, Williams JM, Hosoi H, Houghton PJ, Lawrence JC Jr, Abraham RT
Science (New York, N.Y.) 1997 Jul 4;277(5322):99-101
Science (New York, N.Y.) 1997 Jul 4;277(5322):99-101
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular staining of freshly harvested (top left), untreated (top right), 30-minute LY294002-treated (bottom left), or 30-minute LPS-stimulated (bottom right) normal human peripheral blood cells with Anti-Human CD14 FITC (Product # 11-0149-42) and Anti-Human/Mouse phospho-4E-BP1 (T36/T45) eFluor® 660 using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Total cells were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Impaired Autolysosomal Degradation Determines the Enhanced Immunogenic Potential of IFNalpha-Shaped and SLE Monocytes CD14 + monocytes from healthy donors were cultured for 18 hr with IFNalpha (400 ng/mL) +/- rapam. (1 muMu) as depicted. (A) Levels of HLA-DR and CD86 membrane expression were measured by flow cytometry. A representative result is depicted. Geometric mean fluorescence intensity (GeoMFI) averages are plotted (n = 6). (B) Concentrations of secreted IL6 and TNFalpha measured by ELISA in culture supernatants (n = 3). (C) CFSE-labeled cord blood-naive CD4 + T cells were cultured for 6 days with allogeneic monocytes, previously treated for 18 hr with IFNalpha, +/- rapam., and analyzed for their proliferation. Proliferation index averages of 4 experiments are graphed. Right: histograms of CFSE dilution and size (FS) of CD4 + gated cells. GeoMFI of un-proliferated cells and FS counting are listed on right side of histograms. One representative result is depicted. (D) p-4EBP1 and p-P70S6K expression in freshly isolated monocytes from healthy (n = 5) and SLE (n = 5) donors were analyzed by flow cytometry, and averages of their GeoMFIs are graphed. (E) MLR between SLE monocytes +- rapam. (1 muM) for 1 hr, cocultured with allogeneic CFSE-labeled cord blood-naive CD4 + T cells for 6 days (n = 3). Averages of proliferation indexes as in (C) and averages of % HLADR hi CD86 hi population after cocultures are graphed. (F) Representative histogram of Lysosensor Gre